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Updated: Jun 5, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Handheld hyperspectral imaging dataset of annual sowthistle and little mallow under abiotic stress for machine
Elijah Brunnengraeber1, Tyler Cassidy1, Dylan Woodbridge1
1Department of Biology, Agriculture, and Chemistry, California State University, Monterey Bay, 100 Campus Center, Seaside, CA 93955, USA.
Abstract:
Machine learning has become an increasingly important tool for overcoming agricultural challenges by enabling efficient and consistent classification of crop-related data. Training such supervised models requires high quality labeled datasets. This work presents a dataset consisting of raw and preprocessed hyperspectral imaging (HSI) files capturing reflectance in the visible to near-infrared range (400-1000 nm) from two problematic weed species on California's Central Coast: annual sowthistle (Sonchus oleraceus) and little mallow (Malva parviflora). Hyperspectral imaging provides rich spectral-spatial data cubes that can support the development of deep learning models and autonomous technology for precision weed management. Plants were grown in a greenhouse under five conditions: standard, drought, overwatering, excess fertilizer, and no fertilizer. Custom MATLAB scripts were utilized for preprocessing, including k-means clustering to define regions of interest (ROIs), and extraction of spectral metrics. Data visualization was performed using Wolfram language and MATLAB. The dataset includes both raw and ENVI-formatted hyperspectral cubes and pre-processed MATLAB outputs, supporting spectral feature engineering, benchmark development, and exploratory machine learning workflows for controlled environment stress classification.
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