A Pocketed Immunoblotting Disc for Field-Deployable Profiling of Multiantibody Spectrum
Hong Zhang1, Ruining Liu1, Taiju Chen1
1Department of Laboratory Medicine, Chongqing Center for Clinical Laboratory, Intelligent Diagnostics Key Laboratory of Chongqing Education Commission, Chongqing Academy of Medical Sciences, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing 401147, PR China.
ACS Nano
|June 4, 2026
Summary
A novel pocketed immunoblotting disc assay (PiDa) enables rapid, field-deployable blood group antibody profiling. This innovative method significantly accelerates unexpected antibody identification for emergency medicine, achieving high accuracy in minutes.
Area of Science:
- Biotechnology
- Immunology
- Medical Diagnostics
Background:
- Rapid identification of unexpected blood group antibodies is crucial for emergency medicine.
- Current field-deployable methods are limited by complex procedures and slow results.
- There is a need for a portable and efficient assay for antibody profiling.
Purpose of the Study:
- To develop a pocketed immunoblotting disc assay (PiDa) for rapid, field-deployable profiling of unexpected blood group antibodies.
- To create a microenvironment that accelerates erythrocyte-IgG antibody agglutination.
- To enable quick, multi-antibody signal readout using a portable device.
Main Methods:
- Developed a cotton linter@polyethylene glycol-modified hydrogel sticker to create a 3D microwell mesh for accelerated molecular collisions.
- Constructed an immunoblot array by stamping the hydrogel sticker onto a Blu-ray disc.
- Utilized a pocketed Blu-ray disc driver with a machine learning algorithm for rapid signal readout.
Main Results:
- Achieved ultrafast erythrocyte-IgG antibody agglutination within 2 minutes.
- Successfully profiled 28 unexpected antibody classes simultaneously within 8 minutes using PiDa.
- Demonstrated 100% accuracy in analyzing 150 clinical blood samples, outperforming classical methods by 8 times.
Conclusions:
- The pocketed immunoblotting disc assay (PiDa) offers a rapid, accurate, and field-deployable solution for unexpected antibody profiling.
- PiDa significantly reduces assay time compared to traditional methods, enhancing its utility in emergency settings.
- This portable platform shows great potential for high-throughput biomolecular determination and clinical diagnostics.
