Related Experiment Video

Updated: Jun 5, 2026

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

Chlamydia pneumoniae facilitates its development by recruiting PI4P to inclusion bodies via the Cpn0308-ACBD3-PI4KB

Xiaonan Feng1, Tian'ai Cao1, Xiaohui Jia1

  • 1Key Laboratory of Clinical Laboratory Diagnostics, Institute of Pathogenic Biology and Immunology, Hebei North University, Zhangjiakou, China.

Infection and Immunity
|June 4, 2026
PubMed

Insights

Chlamydia pneumoniae uses its Cpn0308 protein to interact with host ACBD3, recruiting PI4KB to produce PI4P. This process is essential for chlamydial development and infection progression.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Chlamydia pneumoniae (Cpn) is an intracellular pathogen that modifies host cell vacuoles (inclusions) using inclusion membrane proteins (Incs).
  • Cpn0308 is a Cpn Inc that localizes to the inclusion membrane and interacts with host proteins.

Purpose of the Study:

  • To investigate the interaction between Cpn0308 and host protein ACBD3 during Cpn infection.
  • To elucidate the mechanism by which this interaction affects Cpn development and pathogenicity.

Main Methods:

  • Utilized ACBD3 knockout HeLa cells to assess Cpn development.
  • Employed endogenous protein analysis to confirm Cpn0308-ACBD3 interaction during infection.
  • Investigated the role of Phosphatidylinositol 4-kinase 3β (PI4KB) and Phosphatidylinositol 4-phosphate (PI4P) in the Cpn0308-ACBD3 pathway.
  • Used pharmacological inhibition of PI4KB to evaluate its impact on Cpn replication.

Main Results:

  • ACBD3 knockout significantly reduced inclusion size, delayed pathogen development (EB to RB transition), and decreased bacterial load.
  • The Cpn0308-ACBD3 interaction facilitates PI4KB recruitment, enhancing PI4P production at the inclusion.
  • PI4KB inhibition suppressed PI4P synthesis and inhibited Cpn replication.

Conclusions:

  • Cpn0308 interacts with host ACBD3 to recruit PI4KB, promoting PI4P synthesis.
  • This PI4P production is crucial for the development and progression of C. pneumoniae infection.
  • The Cpn0308-ACBD3-PI4KB-PI4P axis represents a key host-pathogen interplay for Cpn survival.
Keywords:
ACBD3C. pneumoniaeCpn0308PI4KBPI4Pinclusion membrane protein

More Related Videos

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription07:18

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Published on: October 13, 2015

Related Concept Videos

Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic of...
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Atypical Pneumonia01:14

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...
Diversity of Protists II01:27

Diversity of Protists II

Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...