Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Standardization Challenges and Breakthroughs in Apolipoprotein B Detection: From Immunoassays to LC-MS/MS.

Mass spectrometry reviews·2026
Same author

A mutational signature for brain metastasis tropism in lung adenocarcinoma.

Discover oncology·2026
Same author

Multicenter comparison of three automated procalcitonin (PCT) assays and commutability evaluation of EQA/PT materials.

Clinica chimica acta; international journal of clinical chemistry·2026
Same author

Establishment of a candidate reference method and verification of reference intervals for serum copper in pregnant and non-pregnant women.

Annals of clinical biochemistry·2026
Same author

Glycated albumin: detection methods and standardization.

Clinica chimica acta; international journal of clinical chemistry·2026
Same author

Hypoxia-Induced Osteopontin-Positive Glioma-Associated Macrophages Facilitate Glioma Mesenchymal Transition via NF-κB Pathway Activation.

Cancer communications (London, England)·2026
Same journal

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
Same journal

Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

Clinical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2026

Biochemical Titration of Glycogen In vitro
07:16

Biochemical Titration of Glycogen In vitro

Published on: November 24, 2013

Standardization Challenges in Glycated Albumin Measurement: Methodological Heterogeneity and Quantification

Hao Zheng1,2,3, Rui Wu1,2,3,4, Li Zhang1,2,3,4

  • 1National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital, Beijing, P.R. China.

Clinical Chemistry
|June 4, 2026
PubMed
Summary
This summary is machine-generated.

Standardizing glycated albumin (GA) measurement is crucial for accurate glycemic monitoring. Comparing methods revealed that targeting total glycated lysine residues, not just Lys-525, provides more accurate GA quantification, especially at higher concentrations.

More Related Videos

Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide
04:50

Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide

Published on: August 4, 2023

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Related Experiment Videos

Last Updated: Jun 5, 2026

Biochemical Titration of Glycogen In vitro
07:16

Biochemical Titration of Glycogen In vitro

Published on: November 24, 2013

Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide
04:50

Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide

Published on: August 4, 2023

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Area of Science:

  • Clinical Chemistry
  • Biomarker Analysis
  • Proteomics

Background:

  • Glycated albumin (GA) is a key biomarker for glycemic status monitoring.
  • Methodological heterogeneity in GA measurement leads to discrepancies, hindering standardization.
  • Existing methods vary in analytical principles and target measurands.

Purpose of the Study:

  • To systematically compare prevailing GA measurement methodologies.
  • To identify a robust reference measurement procedure for widespread standardization.
  • To address quantification discrepancies in glycated albumin assays.

Main Methods:

  • Comparison of targeted bottom-up proteomics (ID-LC-MS/MS), enzymatic assay, and JSCC reference method.
  • Analysis of 129 donor serum specimens and certified reference materials.
  • Examination of GA concentration-dependent glycation kinetics at the Lys-525 site.

Main Results:

  • Optimized proteomics method correlated strongly (r=0.986) with enzymatic and JSCC methods.
  • Site-specific Lys-525 measurement underestimated GA at higher concentrations, showing increasing negative bias.
  • Glycation ratio at Lys-525 declined relative to total glycated lysine residues as overall GA concentration increased.

Conclusions:

  • Standardization requires a precise, universally accepted definition for GA measurands.
  • Quantification of total glycated lysine residues aligns better with biological GA values than site-specific Lys-525 measurement.
  • Adopting total glycated lysine residues as the preferred measurand definition will improve GA detection accuracy and clinical comparability.