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Related Experiment Video

Updated: Jun 6, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Artificial Transmembrane Channels for Selective Ascorbic Acid Transport and Bioorthogonal Signal Transduction.

Linlin Shi¹, Jingjing Ma¹, Linshuang Wang¹

¹State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, School of Pharmaceutical Sciences, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, China.

Angewandte Chemie (International Ed. in English)

|June 4, 2026

Summary

Researchers developed artificial organic nanotubes that form channels for selective ascorbic acid (AA) transport across cell membranes. These channels enable AA to trigger bioorthogonal reactions, converting chemical signals into fluorescence in biomimetic systems.

Keywords:

artificial channels ascorbic acid cyclodextrins supramolecular chemistry transmembrane transport

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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

Published on: June 22, 2022

Area of Science:

Biomaterials Science
Chemical Biology
Nanotechnology

Background:

Ascorbic acid (AA) is vital for physiological functions, with its transport regulated by natural proteins.
Understanding and controlling AA transport is crucial for cellular homeostasis and therapeutic applications.

Purpose of the Study:

To engineer artificial transmembrane channels for selective ascorbic acid transport.
To investigate the potential of these channels in initiating bioorthogonal reactions and signal transduction.

Main Methods:

Construction of discrete covalent organic nanotubes to form artificial transmembrane channels.
X-ray structural analysis to determine channel architecture and pore size.
Vesicle-based fluorescence assays to confirm AA transport and bioorthogonal reaction initiation.

Main Results:

The synthesized organic nanotubes formed stable channels in lipid bilayers with near-nanometer pore sizes.
These channels demonstrated efficient and selective transport of ascorbic acid.
AA transport through the artificial channels successfully initiated bioorthogonal reactions, generating a fluorescent signal.

Conclusions:

Artificial transmembrane channels from covalent organic nanotubes offer a novel method for controlled ascorbic acid transport.
This technology provides a platform for developing responsive biomimetic systems and chemical sensors.