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Updated: Jun 6, 2026
13:24
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
From Sequencing to Treatment: Operational Challenges in Translating Precision Oncology Into Clinical Practice
Miguel Zugman1, Paulo V Campregher1,2, Jeffrey S Ross3,4
1Centro de Oncologia e Hematologia Família Dayan-Daycoval, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Summary
Precision oncology uses next-generation sequencing (NGS) for personalized cancer treatment. Understanding diagnostic workflows and molecular alterations is key to translating genomic insights into improved patient outcomes.
Area of Science:
- Genomic Medicine
- Cancer Biology
- Molecular Diagnostics
Background:
- Precision oncology matches cancer therapies to individual tumor molecular biology.
- Next-generation sequencing (NGS) and biomarker discovery are integral to modern cancer care.
- Comprehensive molecular testing is essential for effective precision oncology.
Purpose of the Study:
- To highlight the importance of understanding diagnostic workflows in precision oncology.
- To discuss how technical and preanalytic variables impact genomic testing.
- To explain the interpretation of genomic alterations and their translation into treatment decisions.
Main Methods:
- Review of next-generation sequencing (NGS) assay designs and their influence on detection.
- Analysis of preanalytic variables affecting molecular testing success and accuracy.
- Examination of interpretation strategies for diverse genomic alterations.
Main Results:
- NGS assay design (breadth, depth, chemistry) affects detectable alterations and sensitivity.
- Preanalytic factors like tissue quality and handling are critical for successful testing.
- Structured reporting and multidisciplinary interpretation aid clinical decision-making.
Conclusions:
- Coordinated integration of molecular diagnostics, clinical interpretation, and healthcare systems is vital for maximizing precision oncology's impact.
- Understanding the entire pathway from specimen acquisition to treatment decision-making improves patient outcomes.
- Effective translation of genomic insights requires a comprehensive approach to molecular testing and interpretation.
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