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Cross-linking with diazirines: Bright sides and blind spots

Andrea Sinz1, Alice Vetrano2, Claudio Iacobucci2

  • 1Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany; Center for Structural Mass Spectrometry, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.

Structure (London, England : 1993)
|June 4, 2026
PubMed
Summary

Diazirine cross-linkers in mass spectrometry are not unselective, as they preferentially react with acidic residues. Ignoring this bias in cross-linking mass spectrometry can lead to inaccurate protein structural models.

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