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Updated: Jun 6, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Digital light processing programs shape-morphing hydrogels into undulating 3D scaffolds supporting corneal limbal

Ioannis Paschalidis¹, François Chatelain², Remy Agniel³

¹Translational Research and Experimental Corneal Surgery (TREX), Hôpital Fondation A. de Rothschild, Paris, France; Université Paris Cité, Inserm, IRSL Institut de Recherche Saint Louis, U1342, Paris, France; CEA IRIG, Grenoble, France.

Acta Biomaterialia

|June 4, 2026

Summary

Researchers developed a simple bioprinting method to create 3D corneal scaffolds with undulating topography. This innovative approach supports corneal epithelial stem cell niche modeling for three weeks, advancing eye research.

Keywords:

Cornea Digital light processing bioprinting Limbal epithelial stem cells Shape morphing hydrogel Silk fibroin

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Last Updated: Jun 6, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

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Published on: September 12, 2014

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Published on: September 29, 2016

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

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Published on: February 11, 2011

Area of Science:

Biomaterials Science
Tissue Engineering
Ophthalmology

Background:

Corneal epithelial homeostasis relies on limbal stem cells in a niche with stromal invaginations.
Existing in vitro limbal niche models are complex and difficult to scale.

Purpose of the Study:

To develop a simple, rapid strategy for fabricating 3D scaffolds mimicking the corneal stem cell niche topography.
To create advanced in vitro corneal models for disease research and preclinical testing.

Main Methods:

Fabrication of 3D scaffolds using a photocrosslinkable bioink (methacrylated collagen, hyaluronic acid, silk fibroin) via Digital Light Processing (DLP).
Spatially controlled heterogeneous crosslinking densities achieved through grayscale UV projection.
Shape-morphing hydrogel concept utilizing differential shrinking dynamics at 37°C to create undulating topography.

Main Results:

A single-step bioprinting process created 3D scaffolds with programmable undulating topography.
Silk fibroin was crucial for shape-morphing and sustained corneal epithelial cell adhesion and growth.
The scaffolds supported epithelial stratification, physiologically relevant mechanotransduction, and apicobasal organization for over three weeks.
Demonstrated progressive epithelial maturation with CK3 expression, tight junctions, and basement membrane deposition.

Conclusions:

Introduced an innovative, single-step bioprinting approach for complex topography scaffolds using DLP and shape-morphing hydrogels.
Engineered advanced corneal models recapitulating the stem cell niche, reducing animal use.
The model sustains long-term cultures and mimics physiological epithelial organization, supporting disease modeling and preclinical applications.