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Plaque formation by Teschen disease virus and the effect of certain associated factors

A H Dardiri, P D Delay

    Canadian Journal of Comparative Medicine and Veterinary Science
    |July 1, 1966
    PubMed

    Insights

    A new plaque assay method was developed for Teschen disease virus, enhancing virus detection and quantification. This standardized technique improves the accuracy of Teschen disease virus diagnosis in primary pig kidney cultures.

    Area of Science:

    • Virology
    • Cell Biology

    Background:

    • Teschen disease virus poses a significant threat to swine health.
    • Accurate and efficient diagnostic methods are crucial for controlling disease outbreaks.

    Purpose of the Study:

    • To develop and standardize a plaque assay for Teschen disease virus.
    • To optimize conditions for plaque formation and virus quantification.

    Main Methods:

    • Utilized primary pig kidney cultures.
    • Incorporated neutral red stain into the agar overlay medium.
    • Standardized virus adsorption time, temperature, inoculum volume, and neutral red concentration.

    Main Results:

    • Maximal plaque counts were achieved with 45-60 minutes of virus adsorption at 28°C and 37°C.
    • Small inoculum volumes enhanced plating efficiency.
    • Neutral red concentration directly influenced plaque number.
    • Plaque counts showed uniformity and a linear relationship with virus concentration.
    • Successful isolation of the virus from infected spinal cord supernatant was demonstrated.

    Conclusions:

    • The developed plaque assay is a reliable and sensitive method for Teschen disease virus detection.
    • The assay allows for accurate quantification of viral titers.
    • This technique facilitates virus isolation and characterization.

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