Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction Note: Surface modification minimizes the toxicity of silver nanoparticles: an in vitro and in vivo study.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026
Same author

Global prediction of antimicrobial resistance burden and trends using machine learning, deep learning, and GenAI.

Annals of medicine and surgery (2012)·2026
Same author

Agentic AI scientists and the rise of virtual laboratories.

Annals of medicine and surgery (2012)·2026
Same author

The transformative impact of AI-enabled AlphaFold 3: evolution, current status, and future prospects in structural biology.

Frontiers in artificial intelligence·2026
Same author

CRISPR-Cas9-mediated therapeutics: Current clinical trials and therapy approval landscape to treat human diseases.

Molecular therapy. Nucleic acids·2026
Same author

Advances in methotrexate nano-formulations to enhance therapeutic efficacy against drug-resistant breast cancer.

Discover nano·2026

Related Experiment Video

Updated: Jun 6, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Understanding the microRNA signature associated with Reye's syndrome.

Ananya Puhan1, Satyajit Tripathy2, Jiban Kumar Behera1

  • 1Department of Zoology, Fakir Mohan University, Vyasa Vihar, Balasore, 756020, Odisha, India.

Protoplasma
|June 4, 2026
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) significantly impact Reye's syndrome development by disrupting liver enzyme metabolism and causing brain and liver swelling. Understanding miRNA roles is key to developing new treatments for this rare childhood illness.

Keywords:
Cerebral edemaHepatomegalyMitochondrial dysfunctionReye’s syndromemiRNAs

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Related Experiment Videos

Last Updated: Jun 6, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pediatrics

Background:

  • Reye's syndrome is a rare, severe childhood illness characterized by brain and liver dysfunction.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and are implicated in disease pathogenesis.
  • Previous research has linked aspirin use in children to Reye's syndrome, leading to public health warnings.

Purpose of the Study:

  • To review the critical role of miRNAs in the development of Reye's syndrome.
  • To explore the influence of miRNAs on hepatic metabolism and their contribution to cerebral edema and hepatomegaly.
  • To highlight the importance of understanding miRNA involvement for future therapeutic strategies.

Main Methods:

  • Literature review of studies on Reye's syndrome and miRNA.
  • Analysis of miRNA's regulatory functions in gene expression.
  • Examination of miRNA's impact on liver enzymes and mitochondrial function.

Main Results:

  • miRNAs play a crucial role in the onset and progression of Reye's syndrome.
  • miRNAs significantly affect liver enzyme metabolism, leading to dysfunction.
  • miRNAs contribute to the pathogenesis of cerebral edema and hepatomegaly in Reye's syndrome.

Conclusions:

  • A comprehensive understanding of miRNA involvement in hepatic metabolism and cerebral edema is essential.
  • Targeting miRNA pathways may offer novel therapeutic approaches for Reye's syndrome.
  • Further research into miRNA-mediated mechanisms is needed for effective treatment development.