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Published on: May 10, 2017
Expression patterns of GPX4 and LINC00618 across disease phases in pediatric B-cell precursor acute lymphoblastic
Maryam Shayanmanesh1,2, Seyed Esmaeil Ahmadi3, Mohammad Faranoush4
1Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.
Background:
Acute lymphoblastic leukemia (ALL) remains a significant challenge in pediatric malignancies, particularly in cases of relapse and treatment resistance. This study aimed to investigate the expression patterns of ferroptosis-related genes, GPX4 and LINC00618, across different disease phases in pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL).
Methods:
This case-control study analyzed 82 samples from pediatric BCP-ALL patients comprising 14 new cases, 29 in remission, and 17 relapsed cases alongside 22 healthy controls. Expression levels of GPX4 and LINC00618 were evaluated using quantitative real-time PCR. The relationship between gene expression and clinical parameters was assessed. Statistical analyses were performed employing parametric or nonparametric tests.
Results:
GPX4 expression was significantly elevated in ALL patients compared to controls (p < 0.01), with notably higher levels in new cases (p < 0.01) and relapsed patients (p < 0.05). Similarly, LINC00618 exhibited significant upregulation in ALL patients (p < 0.05), particularly in new cases (p < 0.01) and relapsed patients (p < 0.05). Both genes demonstrated reduced expression during the remission. External validation using the GSE13159 dataset demonstrated a consistent direction of increased GPX4 expression in ALL samples compared to controls.
Conclusions:
Our findings suggest phase-associated expression patterns of GPX4 and LINC00618 in pediatric BCP-ALL. The consistent direction of GPX4 expression observed in external validation supports its potential relevance in leukemic biology. These results provide a basis for further studies to clarify the role of ferroptosis-related pathways in disease progression and therapeutic response.
