Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
11:08

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Published on: December 7, 2021

Isolation and Characterization of Extracellular Vesicles from Mouse Retina Tissue.

Ishrat Ahmed, Olesia Gololobova, Mohammed Amanullah

    Biorxiv : the Preprint Server for Biology
    |June 5, 2026
    PubMed
    Summary

    This study presents a standardized protocol for isolating extracellular vesicles (EVs) from mouse retinal tissue. This method improves upon cell culture by reflecting in vivo biology for studying retinal EVs.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Correction: Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach.

    Scientific reports·2026
    Same author

    Diabetes Equipment Accessibility Standards 2 Decades Late.

    JAMA ophthalmology·2026
    Same author

    A critical role for mitochondrial dynamics in cigarette smoke condensate-induced RPE senescence.

    Free radical biology & medicine·2026
    Same author

    Case Report: Bilateral optic nerve head masses and retinopathy in a patient with B-cell acute lymphoblastic leukemia receiving CAR T-cell therapy masquerading as fungal endophthalmitis.

    Frontiers in immunology·2026
    Same author

    Clinical Spectrum of Heparan-α-glucosaminide N-acetyltransferase-Associated Nonsyndromic Retinitis Pigmentosa.

    Journal of vitreoretinal diseases·2026
    Same author

    Disease and Participant-Related Correlates of Genetic Testing Completion for Hereditary Eye Disorders in a Cohort of over 1400 Patients.

    Ophthalmology science·2026

    Area of Science:

    • Ophthalmology
    • Cell Biology
    • Biochemistry

    Background:

    • Extracellular vesicles (EVs) play crucial roles in intercellular communication within the retina.
    • Current methods often utilize cell cultures, which may not accurately represent in vivo retinal EV biology.
    • A standardized protocol for isolating EVs from intact retinal tissue is needed.

    Purpose of the Study:

    • To establish a standardized workflow for the isolation and characterization of extracellular vesicles (EVs) from mouse retinal tissue.
    • To provide a method that better reflects in vivo physiological and pathological conditions compared to cell culture models.
    • To ensure adherence to the International Society for Extracellular Vesicles Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines.

    Main Methods:

    More Related Videos

    Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
    09:59

    Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

    Published on: October 11, 2021

    Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
    02:39

    Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

    Published on: April 3, 2011

    Related Experiment Videos

    Last Updated: Jun 6, 2026

    Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
    11:08

    Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

    Published on: December 7, 2021

    Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
    09:59

    Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

    Published on: October 11, 2021

    Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
    02:39

    Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

    Published on: April 3, 2011

    • Isolation of EVs from fresh mouse retinal tissue.
    • Assessment of EV size and concentration using nanoparticle tracking analysis.
    • Characterization of EV marker expression (e.g., CD9, CD63, TSG101) via Western blotting or flow cytometry.
    • Visualization of EVs using electron microscopy.

    Main Results:

    • A reproducible protocol for isolating high-purity EVs from mouse retina was developed.
    • EVs isolated from retinal tissue exhibited characteristic EV markers and size distribution.
    • The protocol allows for comprehensive analysis of EV properties in a biologically relevant context.

    Conclusions:

    • This standardized protocol enables rigorous study of retinal EVs in their native in vivo environment.
    • The method addresses a critical gap in the field, facilitating a deeper understanding of retinal EV function.
    • This approach supports the investigation of EVs in retinal physiology and disease pathogenesis.