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Updated: Jun 6, 2026

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
Published on: November 22, 2024
The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission
Abstract:
Sexual maturation and fertility of the malaria causing parasite, Plasmodium spp., is critical to transmission between hosts. This developmental process involves an intricate gene expression regulatory network that drives gametocyte commitment, development, and the production of male and female gametes. Here, we characterized the role of the CCCH-type zinc-finger protein Plasmodium falciparum Male Development 3 ( Pf MD3) in the formation of gametes. Genetic disruption of Pf MD3 revealed a male fertility defect that decreased infectivity to mosquitoes. Molecular characterization of Pf MD3 revealed its binding to mRNA transcripts associated with male gamete development through a specific RNA sequence. Gametocyte proteins encoded by these Pf MD3-bound transcripts decreased significantly in Pf MD3 knockout parasites, suggesting that Pf MD3 impacts translation. In addition to RNA-binding, Pf MD3 also interacts with proteins involved in mRNA processing and nuclear export that influence translational regulation. This study establishes Pf MD3 as an RNA-binding protein that impacts lineage-specific translation and male gamete fertility.
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Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
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