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Related Concept Videos

Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
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Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Chromatin Packaging01:32

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...

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Updated: Jun 6, 2026

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
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Published on: May 12, 2023

Chromatin architecture sets origin licensing capacity.

Eric J Foss1, Ilana M Nodelman2, Alexa Usenko1

  • 1Division of Translational Sciences and Therapeutics and Human Biology, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA.

Biorxiv : the Preprint Server for Biology
|June 5, 2026
PubMed
Summary
This summary is machine-generated.

Chromatin structure limits DNA replication origin licensing in budding yeast. The chromatin remodeler Isw2 acts as a key inhibitor, controlling helicase loading during the cell cycle.

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Related Experiment Videos

Last Updated: Jun 6, 2026

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

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Published on: May 12, 2023

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Replication origin licensing is crucial for accurate genome duplication.
  • The in vivo regulation of this process by chromatin is not well understood.

Purpose of the Study:

  • To investigate how chromatin architecture regulates replication origin licensing in budding yeast.
  • To identify factors that limit helicase loading during the cell cycle.

Main Methods:

  • MCM-ChEC-seq to track helicase loading dynamics.
  • Analysis of histone modifications (H3K56 acetylation) and chromatin remodelers (Isw2).
  • Genetic manipulation of deacetylases (Hst3, Hst4) and Isw2.

Main Results:

  • Origin licensing occurs in a rapid burst at mitotic exit and plateaus early, despite permissive conditions.
  • Chromatin architecture, specifically H3K56 acetylation, limits MCM loading at the helicase-loading step.
  • The chromatin remodeler Isw2 inhibits licensing by repositioning nucleosomes, restricting helicase loading.

Conclusions:

  • Chromatin architecture at replication origins dictates licensing capacity.
  • Newly replicated chromatin adopts a transient, Isw2-dependent inhibitory state.
  • Genome-wide licensing results from the interplay between chromatin-imposed capacity and cell cycle control.