Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
08:38

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

Polysome Profiling Method for Low-Input Human Postmortem Brain.

Vandana Sharma, Anuj Choudhary, Mrunali Dinesh Dhokne

    Biorxiv : the Preprint Server for Biology
    |June 5, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High-temperature stress alters grain quality, starch granule structure, and gene expression in basmati rice cultivars.

    Plant physiology and biochemistry : PPB·2026
    Same author

    Enhanced chiral discrimination in mass spectrometry with orbital angular momentum beams.

    Science advances·2026
    Same author

    Molecular Characterization of the Progressive Landscape of Depression.

    bioRxiv : the preprint server for biology·2026
    Same author

    Psilocybin Decreases Preference for Large Rewards Accompanied by Increased Activity of Parvalbumin Neurons With Perineuronal Nets in the Medial Prefrontal Cortex.

    The European journal of neuroscience·2026
    Same author

    Deep Eutectic Solvent-Mediated Nucleation Engineering of Bi<sub>2</sub>WO<sub>6</sub> Photocatalysts for Enhanced Visible Light Degradation of Organic Pollutants.

    Langmuir : the ACS journal of surfaces and colloids·2026
    Same author

    BLM regulates MALT1-driven NF-κB signalling and is targetable in B-cell malignancies.

    Cell death & disease·2026
    Same journal

    Layered social competition coordinates reproductive hierarchy formation in ants.

    bioRxiv : the preprint server for biology·2026
    Same journal

    Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

    bioRxiv : the preprint server for biology·2026
    Same journal

    Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

    bioRxiv : the preprint server for biology·2026
    Same journal

    Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

    bioRxiv : the preprint server for biology·2026
    Same journal

    Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

    bioRxiv : the preprint server for biology·2026
    Same journal

    Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

    bioRxiv : the preprint server for biology·2026
    See all related articles

    Polysome profiling was adapted for low-input human postmortem brain tissue, overcoming sample quality challenges. This optimized method enables deeper insights into brain translation and disease mechanisms.

    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Genomics

    Background:

    • Polysome profiling analyzes mRNA-ribosome complexes to assess cellular translation dynamics.
    • Sucrose density gradient centrifugation separates ribosome-bound mRNAs based on sedimentation rate.
    • Existing methods are challenging for low-quality, low-input human postmortem brain samples.

    Purpose of the Study:

    • To develop and optimize a polysome profiling protocol for scarce human postmortem brain tissue.
    • To enable the study of translational regulation in the human brain, particularly in postmortem conditions.

    Main Methods:

    • Implementation of a gradient-maker-free manual sucrose gradient preparation method.
    • Adaptation of the protocol for low-concentration, low-input human postmortem brain samples.

    More Related Videos

    A Chromatin Assay for Human Brain Tissue
    11:31

    A Chromatin Assay for Human Brain Tissue

    Published on: March 21, 2008

    Peering at Brain Polysomes with Atomic Force Microscopy
    08:49

    Peering at Brain Polysomes with Atomic Force Microscopy

    Published on: March 16, 2016

    Related Experiment Videos

    Last Updated: Jun 6, 2026

    Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
    08:38

    Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

    Published on: May 22, 2021

    A Chromatin Assay for Human Brain Tissue
    11:31

    A Chromatin Assay for Human Brain Tissue

    Published on: March 21, 2008

    Peering at Brain Polysomes with Atomic Force Microscopy
    08:49

    Peering at Brain Polysomes with Atomic Force Microscopy

    Published on: March 16, 2016

  • Validation of the protocol on neuronal cell lines and mouse brain tissue.

    • Main Results:

    • Successfully optimized polysome profiling for low-input human postmortem brain tissue.
    • Demonstrated the adaptability of the protocol for neuronal cell lines and mouse brain.
    • Enabled tunable sensitivity for low-input samples using manual gradient preparation.

    Conclusions:

    • The developed polysome profiling protocol overcomes key limitations for human postmortem brain analysis.
    • This advancement facilitates research into translational control in neurological disorders.
    • The protocol's adaptability broadens its utility across various biological samples.