Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

Polysome Profiling Method for Low-Input Human Postmortem Brain.

Vandana Sharma^1,2, Anuj Choudhary², Mrunali Dinesh Dhokne²

¹Department of Zoology and Physiology, University of Wyoming, Laramie, WY, 82071, USA.

Biorxiv : the Preprint Server for Biology

|June 5, 2026

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-temperature stress alters grain quality, starch granule structure, and gene expression in basmati rice cultivars.

Plant physiology and biochemistry : PPB·2026

Same author

Enhanced chiral discrimination in mass spectrometry with orbital angular momentum beams.

Science advances·2026

Same author

Molecular Characterization of the Progressive Landscape of Depression.

bioRxiv : the preprint server for biology·2026

Same author

Psilocybin Decreases Preference for Large Rewards Accompanied by Increased Activity of Parvalbumin Neurons With Perineuronal Nets in the Medial Prefrontal Cortex.

The European journal of neuroscience·2026

Same author

Deep Eutectic Solvent-Mediated Nucleation Engineering of Bi<sub>2</sub>WO<sub>6</sub> Photocatalysts for Enhanced Visible Light Degradation of Organic Pollutants.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

BLM regulates MALT1-driven NF-κB signalling and is targetable in B-cell malignancies.

Cell death & disease·2026

Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026

Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026

Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026

See all related articles

Polysome profiling was adapted for low-input human postmortem brain tissue, overcoming sample quality challenges. This optimized method enables deeper insights into brain translation and disease mechanisms.

Area of Science:

Molecular Biology
Neuroscience
Genomics

Background:

Polysome profiling analyzes mRNA-ribosome complexes to assess cellular translation dynamics.
Sucrose density gradient centrifugation separates ribosome-bound mRNAs based on sedimentation rate.
Existing methods are challenging for low-quality, low-input human postmortem brain samples.

Purpose of the Study:

To develop and optimize a polysome profiling protocol for scarce human postmortem brain tissue.
To enable the study of translational regulation in the human brain, particularly in postmortem conditions.

Main Methods:

Implementation of a gradient-maker-free manual sucrose gradient preparation method.
Adaptation of the protocol for low-concentration, low-input human postmortem brain samples.

More Related Videos

A Chromatin Assay for Human Brain Tissue

A Chromatin Assay for Human Brain Tissue

Published on: March 21, 2008

Peering at Brain Polysomes with Atomic Force Microscopy

Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

Related Experiment Videos

Last Updated: Jun 6, 2026

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

A Chromatin Assay for Human Brain Tissue

A Chromatin Assay for Human Brain Tissue

Published on: March 21, 2008

Peering at Brain Polysomes with Atomic Force Microscopy

Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

Validation of the protocol on neuronal cell lines and mouse brain tissue.

Main Results:

Successfully optimized polysome profiling for low-input human postmortem brain tissue.
Demonstrated the adaptability of the protocol for neuronal cell lines and mouse brain.
Enabled tunable sensitivity for low-input samples using manual gradient preparation.

Conclusions:

The developed polysome profiling protocol overcomes key limitations for human postmortem brain analysis.
This advancement facilitates research into translational control in neurological disorders.
The protocol's adaptability broadens its utility across various biological samples.