Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Aging

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A high-throughput, streamlined cloning protocol to generate guide RNAs for CRISPR activation.

Frontiers in genome editing·2026

Same author

Universal transcriptomic hallmarks of mammalian ageing and mortality.

Nature·2026

Same author

Peroxisomes orchestrate metabolic flexibility and longevity via an interorganelle cascade.

Nature aging·2026

Same author

MAK-2 Kinase Is Required for Extended Longevity and Enhanced Stress Resistance Resulting From Mild Impairment of Mitochondrial Function in isp-1 Mutants.

Aging cell·2026

Same author

Local IFNγ signaling contributes to the regenerative decline of aged alveolar progenitor cells.

bioRxiv : the preprint server for biology·2026

Same author

Mild Mitochondrial Impairment Activates Overlapping Longevity Pathways Converging on the Flavin-Containing Monooxygenase FMO-2.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Multiple Molecular Pathways to Longevity: Opposing Gene Expression Programs Define Distinct Aging Strategies.

Zenith D Rudich^1,2, Jiaxi Guan^1,2, Aura A Tamez González^1,2

¹Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada.

Biorxiv : the Preprint Server for Biology

|June 5, 2026

Summary

This summary is machine-generated.

Targeting aging pathways can protect against neurodegenerative disease. Researchers identified shared longevity genes in worms, offering potential therapeutic targets for healthy aging and age-related diseases.

Keywords:

Aging C. elegans RNA sequencing biological resilience genetics lifespan

More Related Videos

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

Related Experiment Videos

Last Updated: Jun 6, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

Area of Science:

Genetics and Molecular Biology
Aging Research
Neuroscience

Background:

Aging is the primary risk factor for neurodegenerative diseases, but the underlying mechanisms are not fully understood.
Previous research indicates that modulating aging pathways can be neuroprotective in animal models.
Gaining insights into aging processes may reveal novel therapeutic targets for neurodegenerative conditions.

Purpose of the Study:

To identify genes regulated by multiple lifespan-extending pathways using a genomics approach.
To understand how aging impacts gene expression across different longevity pathways.
To discover shared longevity genes that could serve as therapeutic targets.

Main Methods:

Performed RNA sequencing on nine long-lived *C. elegans* mutants representing seven distinct longevity pathways.
Analyzed gene expression overlap and identified distinct longevity groups based on expression patterns.
Filtered for genes consistently modulated across multiple mutants and validated their individual effects on lifespan.

Main Results:

Identified significant overlap in differentially expressed genes among long-lived mutants, revealing three distinct longevity groups.
Discovered 196 upregulated and 62 downregulated genes commonly associated with longevity.
Found that upregulation of specific shared longevity genes enhanced stress resistance and extended lifespan in wild-type worms.

Conclusions:

Shared longevity genes identified through this genomics approach represent potential targets for promoting healthy aging.
These genes may help in developing strategies to mitigate age-onset neurodegenerative diseases.
The study highlights multiple, distinct genetic strategies that can contribute to extended lifespan.