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Published on: October 4, 2019
Pregnenolone biotransformation by a plant-associated Bulbithecium sp. GSBT E3 and in silico insights
Prathyash Ushus Mancheary John1, Siva Kumar Kandula1, Koteswari Peddi1
1Department of Lifesciences (Biotechnology), GITAM School of Science, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh 530045 India.
Abstract:
Pregnenolone was biotransformed by the plant-associated fungus Bulbithecium sp. (GSBT E3) under optimised physicochemical conditions. The resulting metabolites were isolated and structurally characterised using nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS). Three transformed steroidal metabolites were identified: 11β,17α,21-trihydroxypregn-4-ene-3,20-dione (I), 17α-hydroxyprogesterone (III), and pregna-4,17-diene-3,16-dione (IV). Compound II (3-hydroxypregn-5-en-20-one) corresponded to the residual substrate. Among the transformed products, compounds I and IV exhibited structural modifications that were uncommon compared with reported fungal pregnenolone biotransformations. Selected metabolites were further evaluated using Endocrine Disruptome, SwissADME, AdmetSAR 3.0, and PASS online. These findings are preliminary and should be interpreted as hypothesis-generating rather than evidence of biological activity. Overall, Bulbithecium sp. (GSBT E3) functioned as a selective fungal biocatalyst for the transformation of pregnenolone and generated structurally characterised metabolites suitable for future mechanistic and bioactivity studies.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13205-026-04886-w.
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