Novel Electrochemical Immunosensor for Aflatoxin B1 Detection Utilizing Dual Nanoparticles for Dual-Signal...

Area of Science:

• Electrochemistry
• Nanotechnology
• Biosensing

Background:

• Aflatoxin B1 (AFB1) poses significant health risks.
• Accurate and sensitive detection methods are crucial for food safety.
• Existing methods for AFB1 detection often lack sensitivity or speed.

Purpose of the Study:

• To develop a novel electrochemical immunosensor for rapid AFB1 determination.
• To enhance sensor sensitivity and specificity using a unique nanocomposite material.
• To provide a reliable tool for trace-level detection of AFB1.

Main Methods:

• Fabrication of an electrode modified with a nanocomposite of silver nanoparticles (AgNPs), ZIF-90, and multi-walled carbon nanotubes (MWCNTs).
• Electrochemical characterization using techniques like TEM, SEM, XPS, and FT-IR.
• Optimization of sensor parameters for sensitive and specific AFB1 detection.

Main Results:

• The novel nanocomposite demonstrated a dual-signal amplification strategy, improving sensitivity.
• The immunosensor achieved a low quantitative detection limit of 0.05 ng/mL for AFB1.
• The sensor exhibited a wide detection range (0.05-1000 ng/mL) and high recovery rates (93.90-102.15%) in actual sample analysis.

Conclusions:

• The developed electrochemical immunosensor offers a sensitive and rapid method for AFB1 detection.
• The integration of AgNPs, ZIF-90, and MWCNTs provides a significant advancement in immunosensor technology.
• This immunosensor shows great promise as a tool for routine AFB1 monitoring in food safety applications.