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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Updated: Jun 6, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

CRISPR/Cas‑based epigenome editing for osteogenic lineage commitment.

Tengbo Pei1, Weina Yang2, Yutian Lei3

  • 1Department of Medical Laboratory, Xianyang Central Hospital, Xianyang, 712000, Shaanxi, China.

Cell and Tissue Research
|June 5, 2026
PubMed
Summary
This summary is machine-generated.

CRISPR epigenome editing precisely controls mesenchymal stem cell (MSC) differentiation for bone regeneration. This technology reprograms cells to promote bone formation, offering a promising strategy for skeletal repair.

Keywords:
Bone regenerationCRISPR/dCas9Epigenome editingMesenchymal stem cells (MSCs)Osteogenic lineage commitment

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Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
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Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

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Last Updated: Jun 6, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
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Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Epigenetics

Background:

  • Mesenchymal stem cells (MSCs) show limited osteogenic commitment, hindering bone regeneration.
  • Precise control over MSC lineage allocation is crucial for effective skeletal repair therapies.

Purpose of the Study:

  • To explore CRISPR/Cas-based epigenome editing for precise control of osteogenic differentiation in MSCs.
  • To investigate the potential of dCas9-mediated epigenetic modulation for enhancing bone regeneration.

Main Methods:

  • Utilized catalytically inactive Cas9 (dCas9) fused to effectors for locus-specific epigenetic modification.
  • Employed multiplex strategies to activate osteogenic genes (RUNX2, OSX, BMP2) and repress inhibitory loci (PPARG, SOST, DKK1).
  • Investigated various delivery systems including AAV vectors, lipid nanoparticles, and biomaterial scaffolds.

Main Results:

  • Demonstrated successful reshaping of lineage allocation in vitro and in vivo through targeted gene modulation.
  • Preclinical studies in small animals showed significant bone repair.
  • Emerging large-animal data indicate translational potential for skeletal regeneration.

Conclusions:

  • CRISPR/dCas9 epigenome editing offers a powerful, programmable approach to control MSC differentiation for bone regeneration.
  • Advances in delivery, safety, and manufacturing are paving the way for clinical translation of these osteo-epigenetic therapies.
  • Future developments may lead to durable, intelligent therapies for skeletal regeneration.