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Related Experiment Video

Updated: Jun 6, 2026

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
08:30

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Published on: July 12, 2021

Engineering Multicellular Breast Cancer Spheroids in Decellularized Adipose Tissue Hydrogels Using a Microfluidic

Amit Ghosh1, Sourita Ghosh2, Priyanshu Shukla2

  • 1Center for Interdisciplinary Program, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana 502284, India.

ACS Applied Bio Materials
|June 5, 2026
PubMed
Summary

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This summary is machine-generated.

Engineered breast cancer spheroids using decellularized adipose tissue hydrogels and microfluidics mimic the tumor microenvironment. This advanced model enhances preclinical drug screening and personalized cancer organoid development.

Area of Science:

  • Biomaterials Engineering
  • Cancer Biology
  • Microfluidics

Background:

  • Three-dimensional (3D) cancer spheroids are crucial for modeling tumor architecture and microenvironment.
  • Existing spheroid models often lack biomimetic extracellular matrices and stromal complexity.
  • This limits their ability to accurately represent the native tumor microenvironment (TME).

Purpose of the Study:

  • To develop a physiologically relevant breast cancer spheroid model.
  • To integrate decellularized adipose tissue (DAT) hydrogel, microfluidics, and multicellular co-culture.
  • To create a biomaterial-driven platform for advanced cancer research and drug screening.

Main Methods:

  • Fabrication of spheroids using a microfluidic T-junction platform with DAT hydrogel.
Keywords:
Cancer spheroid modelDecellularized adipose tissue (DAT)Microfluidic T-junctionPersonalized medicineTumor microenvironment (TME)

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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

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Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks
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Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

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Related Experiment Videos

Last Updated: Jun 6, 2026

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
08:30

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Published on: July 12, 2021

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
10:51

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Published on: April 23, 2021

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks
06:07

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

Published on: November 2, 2020

  • Co-encapsulation of breast cancer cells (MDA-MB-231) with fibroblasts, monocytes, and endothelial cells.
  • Characterization of spheroid metabolic activity, viability, morphology, gene expression, and drug response.

    • Main Results:

    • Rapid, reproducible, and size-controlled spheroid fabrication (30 spheroids/min, 360-600 μm).
    • Engineered spheroids showed increased metabolic activity (8-fold) and upregulated TME genes (2-40 fold) over 7 days.
    • Multicellular spheroids exhibited enhanced chemoresistance, demonstrating clinically relevant drug response.

    Conclusions:

    • A novel microfluidic platform successfully engineers biomimetic breast cancer spheroids.
    • The model accurately recapitulates TME complexity and stromal interactions.
    • This approach offers a robust platform for preclinical drug screening and personalized cancer organoid development.