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Published on: March 11, 2020
The SlMED25-SlICE2 regulatory module controls cold stress adaptation in tomato
Hongying Guo1, Mingtong Zhai2, Yu Zhang1
1Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an, Shandong 271018, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Summary
Tomato plants
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- The basic helix-loop-helix (bHLH) transcription factors Inducer of CBF Expression (ICEs) regulate plant cold stress responses by controlling cold-responsive (COR) genes.
- The precise mechanisms of ICEs-dependent COR gene regulation are not fully understood.
- The Mediator complex is a conserved coactivator that links transcription factors to RNA polymerase II, but its role in ICEs-mediated transcription is unclear.
Purpose of the Study:
- To investigate the role of the Mediator complex in the transcriptional regulation of cold stress response genes in tomato.
- To elucidate the interaction between SlICE2 and Mediator subunits in mediating cold tolerance.
Main Methods:
- Investigated the role of SlICE2 in cold stress tolerance in tomato (Solanum lycopersicum).
- Examined the physical interaction between SlMED25 (a Mediator subunit) and SlICE2.
- Assessed the coactivator function of SlMED25 in SlICE2-mediated transcriptional activation.
Main Results:
- SlICE2 is crucial for cold stress tolerance in tomato by regulating COR genes.
- SlMED25 physically interacts with SlICE2, and both proteins enhance cold stress responses.
- SlMED25 functions as a transcriptional coactivator, promoting SlICE2 activity during cold adaptation.
Conclusions:
- A novel SlMED25-SlICE2 regulatory module is identified, crucial for plant cold tolerance.
- This module modulates cold stress responses through transcriptional coactivation.
- Provides new insights into the molecular mechanisms of plant cold tolerance.
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