Related Experiment Video
Updated: Jun 7, 2026
An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation
Published on: June 30, 2023
Agrobacterium rhizogenes-mediated stable and transient transformation systems enable rapid functional validation of
Yike Su1, Mengtao Li1, Xubin Wu1
1Laboratory of Fruit Quality Biology/Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
Background:
The established genetic transformation system is essential for gene function research and genetic improvement of horticultural crops. The lack of efficient genetic transformation systems has long been a significant bottleneck for perennial fruit trees such as peach.
Results:
In this study, we provided an Agrobacterium rhizogenes-mediated transformation system by using stem segments of the 'Hanlumi' cultivar (Prunus persica L. Batsch). Through the optimization of explants, selection of the appropriate bacterial strain (MSU440), and determination of the infection concentration (OD600 = 1.0), we obtained transgenic callus and hairy roots with stable GFP expression. Additionally, a rapid transient transformation system was also developed using callus derived from stem and fruit tissues. Both systems were applied to functionally characterize genes involved in the biosynthesis of flavor-related C6 aldehydes. Overexpression of PpLOX1 or PpHPL1 in transgenic callus led to a significant increase in the levels of hexanal and E-2-hexenal, whereas RNAi silencing of PpLOX6 or PpHPL1 in callus resulted in a marked decrease. These results demonstrate that PpLOX1, PpLOX6, and PpHPL1 are key genes in the peach C6 aldehyde pathway.
Conclusions:
The established transformation systems provide effective means for gene function validation and molecular breeding in peach, which have significant implications for the improvement of flavor quality.
