Poly(Lactic Acid) Nanofiber Membrane Loaded with Tannins from Charred Sanguisorbae Radix with Antioxidant and...

Charred Sanguisorbae Radix (CSR) is a well-known traditional Chinese medicine clinically used to treat conditions such as burns and bleeding, with tannins being its main active ingredients. In this study, the tannins (SDT) were isolated and purified from CSR using macroporous resin. Subsequently, SDT was loaded onto a poly(lactic acid) (PLA) nanofiber membrane using electrospinning, and its potential application value as a wound dressing was evaluated. The experimental results demonstrated that the nanofiber membrane loaded with SDT exhibited strong antioxidant activity and favorable antibacterial performance, both of which were enhanced with increasing SDT concentration. Among the tested membranes, the PLA2 membrane with 9% SDT loading (90 mg SDT per 1 g PLA, mass ratio) exhibited excellent biocompatibility and could significantly promote wound closure of L929 fibroblasts in the cell scratch assay. Furthermore, the PLA2 membrane could slowly release gallic acid to continuously exert its therapeutic effect. In summary, the novel SDT-loaded PLA nanofiber membrane shows great potential as a wound dressing, providing a scientific foundation for the further application of CSR in wound healing therapy.