Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lubricious anti-adhesive interface prevents friction, biofilm, and encrustation in long-term indwelling ureteral stents.

Materials today. Bio·2026
Same author

Microfluidic System for Continuous Triacylglycerol Supply with Ethanol as a Cosolvent to Artificial Organelle Models.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Liposome Particle Size Prediction by In-Line Process Analytical Technology (PAT)-Integrated Machine Learning.

Small methods·2026
Same author

Analysis of Interfacial Membrane Polarity of Brain-Related Lipids via Flattened Tetrahedron Phase Diagram.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Thermally Stable Nanoarchitectonics of Bicellar Nanocarriers for Superior Transdermal Delivery of <i>Centella asiatica</i> Extracts.

ACS applied materials & interfaces·2026
Same author

Mechanistic Insights Into the Retention and Separation Mechanism of Poly(Ethylene Glycol)-Modified Short Oligonucleotides in Anion-Exchange Chromatography.

Biotechnology journal·2026

Related Experiment Video

Updated: Jun 8, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

A High-Throughput Screening Platform for Drug-Induced Physicochemical Perturbations in Multidimensional Model

Junghu Lee1, Dabin Lim1, Harutomo Aiba1

  • 1Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.

Nano Letters
|June 6, 2026
PubMed
Summary

We created a microfluidic platform for rapid, detailed analysis of lipid membranes and drug effects. This high-throughput screening method allows for precise mapping of membrane changes caused by compounds.

Keywords:
Drug−Membrane InteractionHigh-Throughput ScreeningIn-Line Process Analysis TechnologyLiposomesModel Membrane

More Related Videos

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Related Experiment Videos

Last Updated: Jun 8, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Area of Science:

  • Biophysics
  • Materials Science
  • Chemical Engineering

Background:

  • Traditional methods for analyzing lipid membranes are low-throughput and time-consuming.
  • Understanding drug-induced membrane perturbations is crucial for drug development.

Purpose of the Study:

  • To develop a microfluidic high-throughput screening (HTS) platform for continuous, in situ physicochemical profiling of model lipid membranes.
  • To enable dynamic liposome synthesis and simultaneous analysis of membrane properties.
  • To map drug-induced perturbations in lipid membranes with high resolution.

Main Methods:

  • Integration of gradient mixing with in-line spectroscopy in a microfluidic device.
  • Dynamically programmable liposome synthesis.
  • Simultaneous analysis of membrane interfacial environment (GP340) and hydrophobic core fluidity (rDPH).
  • Application to study bupivacaine hydrochloride-induced perturbations in ternary model membranes.

Main Results:

  • Generated 786 composition-resolved physicochemical data points in a single day.
  • Enabled high-density mapping of drug-induced membrane perturbations.
  • Demonstrated the platform's capability for detailed analysis of membrane-active compounds.

Conclusions:

  • The developed microfluidic HTS platform overcomes limitations of traditional methods for lipid membrane analysis.
  • The platform facilitates high-resolution, composition-resolved mapping of physicochemical perturbations induced by membrane-active compounds.
  • This technology serves as a valuable tool for drug discovery and membrane biophysics research.