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Related Concept Videos

Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...

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Updated: Jun 8, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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Published on: February 1, 2018

An amperometric l-citrulline biosensor.

Kota Takeda1, Shunsuke Fujii2, Akiko Yoshida3

  • 1National Institute of Advanced Industrial Science and Technology (AIST), Miyagino-ku, Sendai 983-8551, Japan.

Bioelectrochemistry (Amsterdam, Netherlands)
|June 6, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel amperometric biosensor for quantifying plasma l-citrulline, a key biomarker for intestinal function and urea-cycle disorders. The developed sensor offers a rapid and sensitive method for l-citrulline detection, addressing current quantification challenges.

Keywords:
CitrullinemiaEnzyme electrodeMesoporousPoint-of-care testingQuinoneShort bowel syndrome

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Plasma l-citrulline is a crucial biomarker for intestinal function and urea-cycle disorders.
  • Current methods for l-citrulline quantification are often complex and time-consuming.
  • There is a need for rapid and simple quantification of l-citrulline.

Purpose of the Study:

  • To develop and characterize a novel amperometric biosensor for the sensitive detection of l-citrulline.
  • To evaluate the biosensor's performance, including sensitivity, linear range, and interference.
  • To demonstrate the feasibility of using this biosensor for practical citrulline sensing in clinical contexts.

Main Methods:

  • Development of an amperometric biosensor utilizing l-citrulline dehydrogenase (CitDH) immobilized on a modified electrode.
  • Enzyme immobilization on mesoporous carbon electrode with graphene-coated porous silica spheres (G/PSS) and quinoline-5,8-dione (QD).
  • Mediated electron transfer (MET)-type bioelectrocatalysis for l-citrulline oxidation detection.

Main Results:

  • The biosensor demonstrated a linear amperometric response to l-citrulline from 0.040 to 0.787 mM.
  • Achieved a sensitivity of 1.08 ± 0.17 μA cm⁻² mM⁻¹ with minimal interference from l-arginine.
  • The sensor's performance covers clinically relevant concentration ranges for hypercitrullinemia.

Conclusions:

  • This study presents the first amperometric biosensor for l-citrulline detection.
  • The developed biosensor offers a promising electrochemical platform for practical and sensitive citrulline quantification.
  • The findings highlight the potential for improved monitoring of intestinal function and urea-cycle disorders.