An efficient and accurate method for identifying the fertility of PTGMS lines in rice and its application
Ying Zhang1, Wumei Wang1, Dahu Ni1
1Rice Research Institute, Anhui Academy of Agricultural Sciences/Anhui Province Key Laboratory Of Rice Germplasm Innovation and Molecular Improvement, Hefei, 230031, China.
Abstract:
Accurate fertility detection of photothermal-sensitive genic male sterile (PTGMS) lines is the key to reducing the risk of seed production in two-line rice and improving its yield. At present, there are some problems in the detection of fertility of PTGMS lines in cold water pool facilities, such as low levels of automation, difficulty in temperature control, and imperfect equipment. In this study, the existing cold water pool facilities were improved, and an intelligent temperature and light control system with high precision, independent control, real-time monitoring, and remote operation was developed. The fertility changes and the most sensitive stages of PTGMS lines Y58S, PA64S, and 1892S were investigated. The results showed that, regardless of whether the intelligent temperature and light control system was applied, the fertility trends of different PTGMS lines were consistent. The pollen abortion rate and seed setting rate could be affected by both temperature and treatment time, or by treatment time alone. Further studies showed that the pollen abortion rate was positively correlated with temperature and significantly negatively correlated with treatment time, while the selfed-seed set rate was significantly negatively correlated with temperature and significantly positively correlated with treatment time. The most sensitive stages of 1892 S and PA64S were V and III, respectively. Based on the analysis of meteorological data from Hefei over the past 26 years and the critical threshold of 23.5 °C for 6 days identified using the intelligent temperature and light control system, it was preliminarily determined that treatment at 23.5 °C for 6 days could serve as the safety identification condition for PTGMS lines. Furthermore, the safety of PTGMS lines used in local production was evaluated under these conditions. This study provides theoretical and technical support for the efficient and accurate identification of fertility of PTGMS lines in rice.
