Related Experiment Video
Updated: Jun 8, 2026

DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Long-read sequencing technologies and bioinformatics: a new perspective for decoding DNA methylation modifications
Hanjing Hou1,2, Yuanfeng Zhang1,2, Yu Ma1,2
1National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, Beijing, P. R. China.
Background:
DNA methylation is a crucial epigenetic regulatory mechanism in eukaryotes, and its dysregulation is closely associated with numerous diseases. Recent advances in long-read sequencing (LRS) have transformed the ability to comprehensively characterize the methylation modifications in DNA, including the technically difficult genomic regions. However, the translation of this technological potential into reliable biological insights relies heavily on accurate computational analysis of raw signal data. Currently, a growing number of bioinformatic tools have emerged, demonstrating superior performance in LRS-based methylation detection.
Main Body:
Our review first briefly describes the detection principles and technological improvement of LRS, and then provides a comprehensive overview of the existing LRS-based methylation detection tools and related benchmarking studies. We further explore the applications in biomedical research, the current challenges, and the future perspective of LRS-based methylation detection.
Conclusions:
By highlighting the research progress and key issues in the field, this review aims to provide researchers with an essential framework to advance the further development and application of LRS-based methylation detection.
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Sanger Sequencing
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Genomics

