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Updated: Jun 9, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Extended-spectrum β-lactamase-producing Escherichia coli with chromosomally integrated resistance genes isolated from
Michiyo Sugiyama1, Tomoya Morimoto1, Tetsuo Asai1
1Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Abstract:
Carriage of antimicrobial-resistant bacteria in wildlife is a growing public health concern. We isolated an extended-spectrum β-lactamase-producing Escherichia coli from the feces of a wild bear collected in a persimmon orchard in 2025. The bear isolate exhibited resistance to ampicillin, cefazolin, cefotaxime, tetracycline, and chloramphenicol. Whole-genome sequencing revealed that the strain belonged to ST5044-OUT:H29 and harbored the blaCTX-M-55 and other resistance genes integrated into the bacterial genome. Comparative genomic analysis showed close relatedness to isolates from a Vietnamese pig farmer and a Japanese slaughterhouse wastewater, with pairwise distances of 0-80 single-nucleotide polymorphisms. DNA metabarcoding revealed a diet dominated by wild vegetation, with a minor anthropogenic plant component. Thus, wild bears may contribute to the dissemination of antimicrobial-resistant E. coli at the human-wildlife interface.
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