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Related Experiment Video

Updated: Jun 9, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
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Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Effects of Fluorescence-Activated Cell Sorting on Boar Sperm Motility Subpopulations and In Vitro Embryo Development.

Isabel Rodriguez1, Tyler Weide1,2, Caleigh E Grote1

  • 1Department of Animal Science, Iowa State University, Ames, Iowa, USA.

Molecular Reproduction and Development
|June 8, 2026
PubMed
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Translational animal science·2026

Fluorescence-activated cell sorting (FACS) impacts boar sperm motility and reduces embryo development competence in porcine in vitro fertilization. Sorting redistributed sperm motility states, depleting fast sperm and affecting embryo outcomes.

Area of Science:

  • Reproductive biology
  • Animal science
  • Biotechnology

Background:

  • Flow cytometric sorting can isolate specific sperm subpopulations.
  • The effect of sorting on sperm function and embryo development in pigs is not well understood.

Purpose of the Study:

  • To evaluate the impact of fluorescence-activated cell sorting (FACS) on boar sperm motility.
  • To assess the effect of FACS on sperm developmental competence in porcine in vitro fertilization (IVF).

Main Methods:

  • Boar semen was divided into sorted and unsorted (control) groups.
  • Sperm motility and kinematics were analyzed using computer-aided sperm analysis (CASA) with population-level and single-cell data.
  • Oocytes were fertilized with sorted or unsorted sperm, and cleavage and blastocyst development were assessed.
Keywords:
blastocyst developmentfertilization percentagein vitro fertilizationsperm sorting

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Last Updated: Jun 9, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
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Published on: November 28, 2018

Fish Sperm Assessment Using Software and Cooling Devices
07:57

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Published on: July 28, 2018

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Published on: April 21, 2022

Main Results:

  • FACS significantly reduced cleavage and blastocyst rates in porcine IVF compared to controls.
  • Population-level CASA showed modest changes in motility parameters post-sorting.
  • Single-cell CASA revealed sorting redistributed motility states, depleting fast, progressive sperm.

Conclusions:

  • FACS alters the composition of boar sperm motility subpopulations.
  • Sperm sorting negatively impacts post-fertilization developmental competence in porcine IVF.