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Protein-polysaccharide conjugation in food systems: Design principles, material and processing constraints, and
Naeem Ullah1, Maduni Jayahansi Paththuwe Arachchi1, Gafar Babatunde Bamigbade1
1Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Al-Ain, United Arab Emirates.
Protein-polysaccharide conjugation can improve food functionality, but outcomes vary. Moderate modification enhances properties, while excessive conjugation leads to negative effects like aggregation and impaired digestion.
Area of Science:
- Food Science
- Biochemistry
- Materials Science
Background:
- Protein-polysaccharide conjugates are vital for enhancing food functionality.
- Inconsistent outcomes necessitate a deeper understanding of modification processes.
Purpose of the Study:
- To critically review protein-polysaccharide conjugation methods.
- To analyze the process-structure-function relationships in conjugates.
- To identify key indicators for distinguishing optimal modification from over-modification.
Main Methods:
- Review of Maillard-type glycation, enzymatic coupling, chemical cross-linking, and process-intensified approaches.
- Analysis of measurable indicators: degree of grafting, free amino group loss, browning, particle size, ζ-potential, solubility, interfacial behavior, digestibility, and advanced glycation products.
Main Results:
- Moderate conjugation improves hydration, solubility, emulsion stability, bioactive protection, film formation, and printability.
- Excessive modification results in aggregation, browning, lysine blockage, impaired digestion, and sensory deterioration.
Conclusions:
- Application-specific design is crucial for successful protein-polysaccharide conjugation.
- Addressing real-food constraints, scalability, and safety is essential for industrial relevance.
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