Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Higher Levels of <i>BRCA1</i> Gene Methylation in Sporadic Breast Cancer Patients with a Lower Incidence of Recurrence.

Medical sciences (Basel, Switzerland)·2026

Same author

Development of a Sustainable and Practical Natural Deep Eutectic Solvent-based Ultrasound-assisted Microextraction Method for Determining Fipronil and Its Degradation Products in Brewed Coffee.

Journal of separation science·2026

Same author

Semi-solid Extrusion 3D Printing of Chitosan/Carbon Nanotube Nanocomposite Films for Microextraction of Pesticides in Water.

ACS omega·2026

Same author

Prevalence and diagnostic reliability of BRAF, RAS mutations, and RET/PTC rearrangements in a Latin American public health service population with thyroid nodular disease.

PloS one·2025

Same author

Affordable 3D-printed prototype integrated with open-source electronic platform for low-cost polyamide noncoated adsorption-based microextraction.

Analytica chimica acta·2025

Same author

Effervescent-assisted Liquid-phase Microextraction Employing Switchable Hydrophilicity Solvent for the Determination of Cortisol and Testosterone in Oral Fluid by High-Performance Liquid Chromatography-Diode Array Detection.

Journal of separation science·2024

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

A Green and Practical Magnetic Ionic Liquid-Based Microextraction of DNA Using a Low-Cost 3D-Printed Open-Source

Luiz C Ferreira Neto^1,2, Mônica Silva Alves^1,2, Sofia Aquino Monteiro^1,2

¹Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Rio Grande do Sul 90050-170, Brazil.

|June 8, 2026

Summary

A novel 3D-printed magnetic platform enables cost-effective, high-throughput DNA extraction using magnetic ionic liquids. This method is efficient, practical, and suitable for complex samples like whole blood, advancing nucleic acid analysis.

More Related Videos

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance

Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance

Published on: July 19, 2024

Related Experiment Videos

Last Updated: Jun 9, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance

Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance

Published on: July 19, 2024

Area of Science:

Biotechnology
Analytical Chemistry
Materials Science

Background:

Nucleic acid analysis is crucial for disease identification and monitoring.
Efficient DNA extraction is a bottleneck, requiring cost-effective and PCR-compatible methods.
Current methods often lack high-throughput capabilities or are expensive.

Purpose of the Study:

To develop a novel, high-throughput, and cost-effective microextraction strategy for DNA isolation.
To utilize a lab-made, open-source 3D-printed magnetic platform for DNA extraction.
To assess the efficiency, practicality, and sustainability of the proposed method.

Main Methods:

A 3D-printed magnetic platform with 12 magnetic pins was constructed using Arduino for z-axis movement.
Magnetic ionic liquid ([P6,6,6,14]+[Ni-(hfacac)3]-) was employed for DNA extraction (6.5 μL per extraction).
Optimized conditions involved 600 μL sample volume, 25 min extraction time, pH 8, and 10 min desorption in 300 μL Tris-HCl (pH 5.5).

Main Results:

The method demonstrated high efficiency for capturing the BRAF gene with a linear range of 0.005–5 pg/μL.
Relative recoveries ranged from 92.2% to 117.0%, with intraday and interday precision between 5.0–7.3% and 5.9–11.6%, respectively.
The limit of quantification was 0.005 pg/μL, and the method was successfully applied to whole-blood samples.

Conclusions:

The developed low-cost, open-source device with MIL-based Solid-Phase Microextraction (SDME) offers an efficient and accessible DNA extraction alternative.
The platform is customizable, practical (BAGI score 62.5), and sustainable (SPMS score 7.58).
This approach has potential for further full automation in nucleic acid analysis workflows.