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Related Concept Videos

Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

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Related Experiment Video

Updated: Jun 9, 2026

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

Single-cell transcriptomics profiling elucidates RBP-driven metastatic signaling pathways in ER+ breast cancer.

Mingjie Dong1, Xinyu Li1, Songyu Yang1

  • 1College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.

Iscience
|June 8, 2026
PubMed
Summary

This study reveals how RNA-binding proteins drive estrogen receptor-positive breast cancer progression by altering cell behavior and promoting metastasis. Key proteins are linked to cellular remodeling and invasive signaling pathways.

Keywords:
biological sciences

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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

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Last Updated: Jun 9, 2026

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Estrogen receptor-positive (ER+) breast cancer progression involves significant cellular changes.
  • The role of RNA-binding proteins (RBPs) in ER+ breast cancer metastasis is not fully understood.

Purpose of the Study:

  • To create a comprehensive single-cell atlas of ER+ breast cancer progression.
  • To identify RBPs and signaling pathways involved in malignant transformation and metastasis.

Main Methods:

  • Single-cell RNA sequencing of 198,286 cells from normal, primary, and metastatic ER+ breast cancer tissues.
  • Analysis of transcriptional and post-transcriptional changes, cell-cell communication, and functional perturbation experiments.

Main Results:

  • Identified stage-specific reprogramming and upregulation of RBPs (e.g., KRT18, HSPA6, PKP3) associated with cytoskeletal remodeling and stress adaptation.
  • Discovered metastasis-associated signaling (TIMP1-MMP1, TGFB1-COL7A1/MMP1) at invasive fronts.
  • Demonstrated TGF-β enhances invasion, while MMP1/COL7A1 knockdown reduces EMT and invasion.

Conclusions:

  • RNA-binding proteins play a critical role in ER+ breast cancer progression and metastasis.
  • Specific RBP-associated programs link cellular state transitions to invasive signaling.
  • Targeting pathways like TGF-β, MMP1, and COL7A1 may offer therapeutic strategies.