Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Single-Chain Light-Activatable Transcriptional Reporter for Fluorescently Tagging Mammalian Cells In Vitro.

Chembiochem : a European journal of chemical biology·2026
Same author

The kinetics and mobility of a ParA ATPase drive carboxysome distribution in <i>Halothiobacillus neapolitanus</i>.

bioRxiv : the preprint server for biology·2026
Same author

A novel directed evolution platform for engineering chemically gated protein switches.

Chemical communications (Cambridge, England)·2026
Same author

Library-on-Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides.

Chembiochem : a European journal of chemical biology·2026
Same author

A chemogenetic approach for temporal and cell-specific activation of endogenous GPCRs in vivo.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Principles and Design of Molecular Tools for Sensing and Perturbing Cell Surface Receptor Activity.

Chemical reviews·2025
Same journal

Inositol Thiophosphates as Inhibitors of Mammalian, Plant, and Fungal Phytases.

ACS chemical biology·2026
Same journal

Synthesis and Characterization of the Spectroscopic and Imaging Utilities of Two Indole-Based Cyan Fluorescent Nucleoside Analogues.

ACS chemical biology·2026
Same journal

Indole Ring Expansion and Rearrangement-Enabled Quinoline Scaffold Formation in the Biosynthesis of the Antitumor Monoterpene Indole Alkaloid Camptothecin.

ACS chemical biology·2026
Same journal

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

ACS chemical biology·2026
Same journal

Development of Next-Generation Fluoroacetamidine-Containing Activity-Based Probes for the Selective Labeling of the Protein Arginine Deiminases (PADs).

ACS chemical biology·2026
Same journal

Spectroelectrochemical Insight into Reaction Mechanisms of Cell-Penetrating Peptides on Charged Membrane Surfaces.

ACS chemical biology·2026
See all related articles

Related Experiment Video

Updated: Jun 9, 2026

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

SENSIT, a Modular Single-Chain Fluorescent Integrator Platform for GPCR Ligands.

Aubrey Putansu1,2, Isabel Solowiej1,2, Hannah Rivett-Trznadel1,2

  • 1Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, United States.

ACS Chemical Biology
|June 8, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new tool, the single-chain expressing neurotransmitter sensing integrator tool (SENSIT), to map neurotransmitter release in the brain. This platform enables high-resolution whole-brain mapping of specific neurotransmitters like epinephrine and norepinephrine.

More Related Videos

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Related Experiment Videos

Last Updated: Jun 9, 2026

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biotechnology

Background:

  • G-protein-coupled receptors (GPCRs) are crucial for neurotransmission but visualizing their activity across large brain volumes at high resolution is challenging.
  • Existing methods struggle to provide detailed insights into neurotransmitter release dynamics and localization.

Purpose of the Study:

  • To develop a novel modular platform for high-resolution neurotransmitter sensing across large brain volumes.
  • To create specific reporters for catecholamines and acetylcholine using the developed platform.
  • To enable generalized sensor design for diverse neurotransmitters for potential whole-brain mapping.

Main Methods:

  • Designed a modular platform named single-chain expressing neurotransmitter sensing integrator tool (SENSIT) utilizing bifunctional chimeric nanobodies.
  • Applied SENSIT to create reporters for epinephrine and norepinephrine with high selectivity.
  • Developed a muscarinic acetylcholine receptor 2 (CHRM2)-based sensor and chimeric miniG proteins to demonstrate platform modularity.

Main Results:

  • Epinephrine and norepinephrine reporters demonstrated 20-fold and 5-fold selectivity, respectively, over other catecholamines.
  • Successfully developed a CHRM2-based sensor, showcasing the platform's versatility.
  • The SENSIT platform represents the first single-chain integrator sensor capable of distinguishing between epinephrine and norepinephrine.

Conclusions:

  • The SENSIT platform offers a powerful, modular approach for high-resolution neurotransmitter sensing.
  • This technology enables the distinction of specific catecholamines and has potential for broad application in neuroscience research.
  • SENSIT paves the way for generalized integrator sensor design and whole-brain mapping of neurotransmitter activity.