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Updated: Jun 10, 2026
08:30
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
Published on: March 4, 2020
Designing complex organoids through developmental principles
Jonathan A Brassard1, James M Wells1
1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA; Center for Stem Cell & Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Cell Stem Cell
|June 8, 2026
Summary
Next-generation organoids use developmental biology principles to create more complex models. These advanced human pluripotent stem cell (hPSC)-derived organoids better mimic human development and disease for research.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Organoid Technology
Background:
- Human pluripotent stem cells (hPSCs) are differentiated into organoids, modeling human development and disease.
- First-generation organoids lacked cellular diversity crucial for studying normal and pathological processes.
Purpose of the Study:
- To discuss advances in creating next-generation organoids with increased cellular complexity.
- To highlight methods for better reproducing in vivo organogenesis processes.
- To explore engineering technologies for controlled in vitro development.
Main Methods:
- Leveraging principles of developmental biology for directed differentiation of hPSCs.
- Focusing on reconstructing cellular diversity within organoids.
- Investigating tissue-tissue interactions and specialized cell addition.
- Utilizing engineering technologies to guide cell assembly.
Main Results:
- Next-generation organoids exhibit enhanced cellular complexity compared to earlier models.
- Improved recapitulation of developmental processes during organogenesis in vitro.
- Demonstrated importance of tissue interactions and cell type incorporation.
Conclusions:
- Next-generation organoids offer more comprehensive models of human development and disease.
- Advances in cellular diversity and engineering enhance organoid fidelity.
- These improved models hold significant potential for future research.
