Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 10, 2026

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

OrgLine: A versatile pipeline for organoid morphometry using detector-guided prompts.

Xun Deng¹, Xinyu Hao², Thomas Herget³

¹The Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China; University of Chinese Academy of Sciences, Beijing, China.

Cell Reports Methods

|June 8, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The impact of mobile payment on rice farmers' purchasing behavior of new rice varieties: a case study of rice cultivation in Jiangxi Province.

Frontiers in nutrition·2026

Same author

Electrochemical Cascade Cyclization of Sulfoxonium Ylides with Unactivated Alkenes.

The Journal of organic chemistry·2026

Same author

A multi-objective optimization framework for planning electric vehicle charging infrastructure incorporating traffic demand, cost, and equity considerations.

Scientific reports·2026

Same author

EMD-HVG: a normalization-independent method for highly variable gene selection based on Earth mover's distance.

BMC bioinformatics·2026

Same author

Soil Region Segmentation and Visual Whiteness Analysis in Cold-Region Rice Seedbeds Based on Improved DAC-UNet.

Plants (Basel, Switzerland)·2026

Same author

TIME: A Taylor-Inspired Mixed-Effects Model for IDH Prediction.

IEEE transactions on bio-medical engineering·2026

OrgLine is a new quantitative analysis pipeline for patient-derived organoids. This tool automates the analysis of organoid morphology from bright-field images, improving disease modeling and drug development.

Area of Science:

Biotechnology
Bioinformatics
Cell Biology

Background:

Patient-derived organoids are vital tools in disease modeling, drug development, and precision medicine.
Manual analysis of organoids in 3D culture is challenging and prone to artifacts in bright-field images.
Quantitative assessment of organoid morphology is crucial but complicated by imaging limitations.

Purpose of the Study:

To develop an automated quantitative analysis pipeline for patient-derived organoids.
To address the limitations of manual workflows and image artifacts in organoid analysis.
To enable accurate morphological characterization and segmentation of organoids.

Main Methods:

Development of OrgLine, a quantitative analysis pipeline for organoids.

Keywords:

CP: imaging CP: stem cell automation bright-field images cultivation organoids pipeline pre-trained quantitative segmentation stem cell

More Related Videos

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer

Published on: January 5, 2024

A High-Throughput Platform for Culture and 3D Imaging of Organoids

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Published on: October 14, 2022

Related Experiment Videos

Last Updated: Jun 10, 2026

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer

Published on: January 5, 2024

A High-Throughput Platform for Culture and 3D Imaging of Organoids

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Published on: October 14, 2022

Utilizing a large, curated dataset of bright-field organoid images.

Implementation of a detector coupled with a prompt-guided segmentation module.

Main Results:

OrgLine accurately recognizes organoids in bright-field images.
The pipeline enables quantitative characterization of morphological phenotypes across developmental stages.
Accurate instance segmentation for downstream morphometric assessment is achieved.

Conclusions:

OrgLine facilitates more automated and quantitative organoid cultivation workflows.
The pipeline enhances the utility of patient-derived organoids in research and clinical applications.
OrgLine improves the reliability and efficiency of organoid analysis.