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Updated: Jun 10, 2026

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Published on: November 23, 2012
Complete genome sequence of Streptococcus lingualis isolated from subgingival biofilm
Seok Bin Yang1,2, Doyun Ku1,2, Kyu Hwan Kwack1
1Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
Objective:
Streptococcus lingualis is a recently described oral streptococcal species; however, genomic information for this species remains limited, with only a single complete genome currently available for the type strain S5ᵀ. The objective of this study was to generate a high-quality complete genome sequence of a subgingival biofilm-derived S. lingualis isolate and expand the genomic resources available for this species.
Data Description:
The S. lingualis strain KHUD_012 was isolated from subgingival biofilm of a healthy adult and sequenced using the PacBio Sequel IIe platform. A single SMRT cell produced 144,821 HiFi reads, which were assembled de novo using the IPA HiFi genome assembler v2.0. The complete genome consists of a single circular chromosome of 2,115,610 bp with a G + C content of 42.0%, encoding 1,956 protein-coding genes, four rRNA operons, and 62 tRNAs. BUSCO analysis indicated 99.5% completeness. Pairwise whole-genome comparisons with closely related type strains showed that KHUD_012 was most closely related to S. lingualis S5ᵀ, with an OrthoANI value of 96.44%, and Parsnp-based core-genome SNP analysis supported this phylogenetic placement. Comparative genomic analysis with S5ᵀ identified 1,717 shared core gene clusters and 198 unique gene clusters in KHUD_012, many of which were functionally uncharacterized.
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