Related Experiment Video

Updated: Jun 10, 2026

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
07:47

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

Published on: September 15, 2023

A multi-omics study reveals pathway-level insights and predictive biomarkers in pediatric TB

Zaynab Mousavian1,2,3, Mark R Segal4, Roger I Calderon5

  • 1Department of Global Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA. zaynab.mousavian@ki.se.

Clinical Proteomics
|June 9, 2026
PubMed

Insights

Diagnosing childhood tuberculosis (TB) is challenging. Integrating proteomics and metabolomics data shows potential, with proteomics alone outperforming other methods for accurate pediatric TB classification.

Area of Science:

  • Pediatric infectious diseases
  • Multi-omics research
  • Biomarker discovery

Background:

  • Tuberculosis (TB) significantly impacts children globally, with over a million cases annually.
  • Diagnostic challenges hinder timely treatment for pediatric TB patients.
  • Accurate diagnosis is crucial for effective management of childhood TB.

Purpose of the Study:

  • To investigate the utility of multi-omics analysis for pediatric tuberculosis (TB) diagnosis.
  • To identify novel immune and metabolic biomarkers for childhood TB.
  • To compare the diagnostic performance of integrated omics data versus single-omics data.

Main Methods:

  • Plasma proteomics and metabolomics data were integrated from children with presumptive TB in high-burden countries.
  • Pathway enrichment analysis (multiGSEA) identified key immune and metabolic pathways.
  • Diagnostic biomarker discovery utilized mixOmics and multiview approaches.

Main Results:

  • Data integration uniquely identified pathways like PTEN/RUNX2 regulation and arginine/proline metabolism.
  • Proteomics data alone demonstrated superior performance in classifying confirmed TB versus unlikely TB in children compared to metabolomics.
  • Multi-omics signatures showed marginal improvement over single-omics models for pediatric TB diagnosis.

Conclusions:

  • Combining complementary molecular data layers enhances understanding of pediatric TB disease mechanisms.
  • Proteomics holds significant promise for improving the accuracy of diagnosing TB in children.
  • Further research into multi-omics approaches can refine diagnostic strategies for pediatric TB.
Abstract

Related Concept Videos

Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Tuberculosis01:23

Tuberculosis

Tuberculosis (TB) remains a significant global health concern, primarily targeting the lungs and spreading through airborne transmission. Infection begins when aerosolized droplet nuclei, expelled by an individual with active TB, are inhaled by another person. These microscopic particles carry Mycobacterium tuberculosis, the causative agent of TB. Upon reaching the alveoli, the bacilli are engulfed by alveolar macrophages. However, due to their specialized lipid-rich cell wall, these pathogens...
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories: