Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Cell Wall02:43

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.Collenchyma and sclerenchyma cells, on the other hand, mainly occur in the outer layers of a plant's stems and leaves. These cells provide the plant with strength and support by either partially thickening their primary cell wall (i.e., collenchyma), or depositing a...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Microbe-Plant Interactions01:09

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations of thyroid hormones with metabolic syndrome and coronary artery stenosis in euthyroid individuals: A cross-sectional study.

Medicine·2026
Same author

Potent Cytotoxic Tumor-Infiltrating Lymphocytes Can Be Generated from Immune-Excluded Chondrosarcomas Using Regulatable Membrane-Bound IL15.

Cancer immunology research·2026
Same author

[Retracted] Reduced expression of Snail decreases breast cancer cell motility by downregulating the expression and inhibiting the activity of RhoA GTPase.

Oncology letters·2025
Same author

Survival Outcomes in Upstaged Cutaneous Head and Neck Melanoma With Negative Sentinel Lymph Node Biopsy: A Retrospective Analysis.

Ear, nose, & throat journal·2025
Same author

Mimicry of molecular glues using dual covalent chimeras.

Nature communications·2025
Same author

Wheat domestication alters root metabolic functions to drive the assembly of endophytic bacteria.

The Plant journal : for cell and molecular biology·2024

Related Experiment Video

Updated: Jun 10, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
11:51

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

An extracellular miRNA reshapes cell wall integrity to prime systemic immunity in tomato.

Yue Qiao1, Yu Xia1, Jianfei Shi2

  • 1Shanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Molecular Plant
|June 9, 2026
PubMed
Summary

Extracellular plant small RNAs (sRNAs) act as mobile immune signals. Apoplastic miR408b targets SlGAUT12, enhancing plant immunity by remodeling cell walls and activating damage-associated molecular pattern-triggered immunity.

Keywords:
Phytophthoraapoplastcell wall integritymiRNAsystemic immunitytomato

More Related Videos

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Related Experiment Videos

Last Updated: Jun 10, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
11:51

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Area of Science:

  • Plant molecular biology
  • Plant pathology
  • RNA biology

Background:

  • Plant small RNAs (sRNAs) regulate gene expression and mediate inter-kingdom RNA interference.
  • The role of extracellular sRNAs in modulating plant immunity via host-intrinsic mechanisms is largely unknown.

Purpose of the Study:

  • To investigate the function of apoplastic small RNAs in plant immunity.
  • To identify specific sRNAs involved in plant-pathogen interactions and their mechanisms of action.

Main Methods:

  • Systematic profiling of apoplastic sRNAs in tomato during Phytophthora capsici infection.
  • Identification of target genes and analysis of their role in plant immunity.
  • Investigation of systemic movement and signaling pathways of identified sRNAs.

Main Results:

  • Specific sRNAs, notably miR408b, were selectively enriched in the tomato apoplast upon pathogen invasion.
  • Apoplastic miR408b moved systemically and targeted SlGAUT12, a negative regulator of immunity.
  • miR408b-induced SlGAUT12 silencing compromised cell wall integrity, promoting oligogalacturonide accumulation and activating damage-associated molecular pattern-triggered immunity.

Conclusions:

  • Apoplastic miRNAs serve as mobile immune signals coordinating systemic plant immunity.
  • This mechanism involves cell wall remodeling and damage-associated molecular pattern signaling.
  • Identified molecular targets offer potential for RNA-based disease control strategies.