Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Antihypertensive Drugs: Vasodilators01:23

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
Paracrine Signaling01:21

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
Antianginal Drugs: Nitrates and β-Blockers01:16

Antianginal Drugs: Nitrates and β-Blockers

In cardiovascular health, antianginal drugs combat angina pectoris — a condition marked by chest pain owing to diminished blood flow to the heart.
Organic nitrates,  such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...
Types of Signaling Molecules01:32

Types of Signaling Molecules

In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small peptides, nucleotides, steroids, fatty acid derivatives, and dissolved gases such as nitric oxide. Some signaling molecules diffuse through the plasma membrane to act locally between neighboring cells or travel long distances. Others remain attached to the cell surface, transmitting information to other cells only when they make contact. In some...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unlocking Interfacial Binder Chemistry for Efficient Li<sup>+</sup> Desolvation in 3C-Rate Lithium Metal Pouch Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

From disordered dots to coherent pixels: superlattice ordering enables high-performance perovskite LEDs.

Science bulletin·2026
Same author

Day-Long Persistent Luminescence in Intrinsically Integrated Donor-Acceptor Carbon Dots Enabled by Defect-Mediated Charge Trapping.

Angewandte Chemie (International ed. in English)·2026
Same author

Highly Dissymmetric and Multicolor Circularly Polarized Organic Hyperafterglow.

Angewandte Chemie (International ed. in English)·2026
Same author

Advanced Electrolyte Materials Design for High-Energy Lithium Metal Batteries Beyond 500 Wh Kg<sup>-1</sup>.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

An SMC-domain protein regulates eupyrene spermiogenesis and sperm migration in a cosmopolitan insect, Plutella xylostella.

Journal of insect physiology·2026

Related Experiment Video

Updated: Jun 10, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Nitric Oxide Tunes Secreted Metabolite Bioactivity.

Zachery R Lonergan1,2, Sarah L Weisflog1, Matthew Scurria3

  • 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Molecular Microbiology
|June 9, 2026
PubMed
Summary
This summary is machine-generated.

Nitric oxide (NO) transforms microbial metabolites, reducing antibiotic effectiveness against competitors like Staphylococcus aureus. This NO-mediated chemical reaction also causes toxicity in Pseudomonas aeruginosa.

Keywords:
pseudomonasnitric oxidephenazinepyocyanin

More Related Videos

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Related Experiment Videos

Last Updated: Jun 10, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Area of Science:

  • Microbiology
  • Chemical Biology
  • Biochemistry

Background:

  • Nitric oxide (NO) is a crucial signaling molecule with widespread physiological and pathological roles.
  • Understanding NO's reactivity with cellular and secreted small molecules is vital for assessing its biological impact.
  • The interaction of NO with microbially-derived secreted small molecules, like phenazines, remains underexplored.

Purpose of the Study:

  • To investigate the reactivity of NO with phenazine metabolites.
  • To determine the impact of NO-mediated phenazine modification on their antibiotic activity.
  • To elucidate the consequences of NO-phenazine interactions on microbial viability and interspecies interactions.

Main Methods:

  • Utilized Pseudomonas aeruginosa as a model organism for phenazine production.
  • Characterized the chemical products formed from the reaction of NO with specific phenazines.
  • Assessed the antibiotic properties of native and modified phenazines against Staphylococcus aureus.
  • Evaluated the viability of P. aeruginosa and S. aureus in the presence of NO and phenazines.

Main Results:

  • NO reacts with specific phenazines to form stable, distinct chemical products.
  • These NO-modified phenazines exhibit significantly reduced antibiotic activity against S. aureus.
  • P. aeruginosa viability rapidly decreases upon reaction of phenazines with NO, independent of S. aureus presence.
  • S. aureus demonstrates resistance to the NO-modified phenazines, suggesting a specific toxicity mechanism.

Conclusions:

  • NO can chemically transform secreted microbial metabolites, altering their functional properties.
  • NO-mediated modification of phenazines attenuates their antibiotic capabilities.
  • NO plays a role in modulating microbial interactions by altering metabolite activity and inducing specific toxicities.