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Updated: Jun 10, 2026

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration
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Intramyocellular Lipid Reduction During Hindlimb Unloading Assessed by In Vivo 1H-MRS.

Hiroyuki Takashima1,2, Hinako Notoh3, Rui Imamura2,4

  • 1Department of Biomedical Science and Engineering, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

NMR in Biomedicine
|June 9, 2026
PubMed
Summary

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This summary is machine-generated.

Hindlimb unloading reduced intramyocellular lipids (IMCL) in rat skeletal muscle. Extramyocellular lipids (EMCL) increased in the plantaris muscle, showing muscle lipid alterations during disuse.

Area of Science:

  • Biomedical Engineering
  • Skeletal Muscle Physiology
  • Metabolic Research

Background:

  • Skeletal muscle lipid content, including intramyocellular lipids (IMCL) and extramyocellular lipids (EMCL), is crucial for muscle function and metabolic health.
  • Hindlimb unloading is a common model to study muscle atrophy and metabolic changes due to disuse.
  • Understanding lipid dynamics in skeletal muscle during unloading is vital for developing countermeasures against muscle deconditioning.

Purpose of the Study:

  • To investigate longitudinal changes in IMCL and EMCL in rat skeletal muscle during a 14-day hindlimb unloading period.
  • To assess the utility of in vivo proton magnetic resonance spectroscopy (1H-MRS) for noninvasively monitoring muscle lipid content.
  • To differentiate lipid changes in different muscle types (tibialis anterior and plantaris) under disuse conditions.
Keywords:
1H‐MRSextramyocellular lipidhindlimb unloadingintramyocellular lipidskeletal muscle

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Main Methods:

  • Male Wistar rats underwent 14 days of hindlimb unloading.
  • In vivo proton magnetic resonance spectroscopy (1H-MRS) was used to measure IMCL and EMCL in the tibialis anterior and plantaris muscles.
  • Measurements were taken at baseline and after the unloading period. Control animals were analyzed under standard housing conditions.
  • Lipid quantification was performed using LCModel analysis.

Main Results:

  • Hindlimb unloading led to a significant decrease in IMCL content in both the tibialis anterior and plantaris muscles.
  • EMCL levels significantly increased in the plantaris muscle but showed no significant change in the tibialis anterior muscle.
  • Control animals exhibited no consistent directional changes in IMCL or EMCL over the same time frame.
  • 1H-MRS provided longitudinal in vivo data on muscle lipid alterations.

Conclusions:

  • Hindlimb unloading induces a reduction in skeletal muscle IMCL content.
  • EMCL alterations were observed in the plantaris muscle, though these findings require cautious interpretation.
  • 1H-MRS is a valuable noninvasive tool for assessing muscle lipid dynamics during disuse and other physiological conditions.
  • These findings contribute to understanding the metabolic adaptations of skeletal muscle to unloading.