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Related Experiment Video

Updated: Jun 10, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

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Published on: April 22, 2016

Reuse of the ReCell Enzyme Does Not Decrease the Density of Spray Cell Suspension While Significantly Reducing Costs.

Derek M Culnan^1,2, Alexandria Gudeman², Jay Coates³

¹Mississippi Burn, Hand, and Reconstruction Center, Mississippi Baptist Medical Center, Jackson, MS, United States.

Journal of Burn Care & Research : Official Publication of the American Burn Association

|June 9, 2026

Summary

This summary is machine-generated.

Repeatedly using the enzyme from one Spray Cell Suspension (SCS) kit for skin grafting significantly reduces costs without impacting cell yield or graft outcomes. This cost-effective method saves hospitals substantial amounts.

Keywords:

burns decreasing costs skin grafting spray cell suspension

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Area of Science:

Regenerative Medicine
Biotechnology
Dermatology

Background:

Spray Cell Suspension (SCS) is a common method for skin grafting.
The high cost of specialized kits for SCS presents a significant financial burden.
Exploring cost-effective alternatives for SCS preparation is crucial for widespread adoption.

Purpose of the Study:

To evaluate the feasibility and cost-effectiveness of repeatedly using the enzyme from a single ReCell kit for Spray Cell Suspension (SCS).
To assess the impact of repeated enzyme use on cell counts, graft outcomes, and hospital charges.
To determine if enzyme reuse affects the quality and efficacy of SCS in skin grafting.

Main Methods:

One ReCell kit enzyme was reused across multiple skin grafting surgeries (9 surgeries, 6 patients).
Cell dosage was meticulously counted, and clinical outcomes were tracked.
Statistical analyses (linear regression, ANOVA, Student's T-test) were employed to assess cell density and differences in enzyme use.

Main Results:

No significant decrease in cell density was observed with repeated enzyme use (up to 14 biopsies).
Clinical outcomes showed 95% wound closure within 7-21 days, with no compromise due to enzyme reuse.
The reuse of one enzyme prevented the purchase of 19 ReCell kits, saving approximately $130,625 in one month.

Conclusions:

Repeated use of the SCS enzyme is a safe, feasible, and cost-effective strategy for augmenting skin grafting.
This approach significantly reduces hospital expenditures without compromising therapeutic efficacy.
Enzyme reuse offers a practical solution to the high cost associated with SCS preparation.