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Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

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Related Experiment Video

Updated: Jun 10, 2026

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model
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Published on: March 9, 2022

Designer Dynamic DNA Nanoaggregate in Living Cell for Mitochondrial Energy Restriction.

Ruijia Deng1, Jing Sheng1, Ben Niu1

  • 1Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 9, 2026
PubMed
Summary

A novel DNA nanoaggregate system, Tech-tetrahedron, precisely targets mitochondria to restrict cellular energy production. This approach significantly inhibits tumor growth and increases apoptosis, offering new avenues for cancer therapy.

Keywords:
DNA tetrahedronartificial nanoaggregatesmitochondrial energy restrictiontelomerase‐responsive assembly

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High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • Controlling artificial intracellular molecule assembly at the subcellular level is difficult.
  • Existing methods lack precise spatiotemporal control for modulating cellular processes.

Purpose of the Study:

  • To develop a DNA nanoaggregate system for spatiotemporal mitochondrial energy restriction.
  • To investigate the potential of Tech-tetrahedron for targeted cancer therapy.

Main Methods:

  • Designed a trinity-functionalized DNA tetrahedron (Tech-tetrahedron) with navigation, enzymatic control, and self-assembly functions.
  • Utilized triphenylphosphine for mitochondrial anchoring and a telomerase-gated latch for initiator release.
  • Triggered catalytic hairpin self-assembly to form DNA nanoaggregates that disrupt mitochondrial function.

Main Results:

  • Tech-tetrahedron successfully anchored to mitochondria and initiated self-assembly.
  • Disruption of mitochondrial-cytoplasmic exchange led to impaired respiration and reduced ATP production.
  • Observed a 24.67% increase in tumor apoptosis and 63.16% tumor growth inhibition.

Conclusions:

  • Tech-tetrahedron provides a precise strategy for subcellular energy intervention.
  • This system demonstrates transformative potential for targeted cellular regulation via artificial nanoaggregates.
  • The findings highlight a feedback loop involving polycystin-1 and potential interference with nicotinamide nucleotide transhydrogenase activity.