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Related Experiment Video

Updated: Jun 10, 2026

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
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Published on: December 7, 2010

Amidoxime-Based Near-Infrared Fluorescent Sensor for Highly Sensitive Uranium Detection in Living Systems.

Qi Wang1, Qiwen Sun2, Mingxuan Wang1

  • 1State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.

Analytical Chemistry
|June 9, 2026
PubMed
Summary

A new fluorescent probe, NRhB-AO, enables rapid and sensitive detection of internal uranium contamination. This breakthrough aids in timely medical treatment for individuals exposed to uranium, improving health outcomes.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Radiochemistry

Background:

  • Uranium contamination poses significant health risks, necessitating accurate biological detection methods.
  • Current methods lack the speed and precision required for effective emergency medical treatment of internal uranium exposure.
  • Developing a sensitive and specific clinical agent for uranyl monitoring is critical for prompt diagnosis and care.

Purpose of the Study:

  • To develop a novel fluorescent probe for sensitive and rapid in vivo detection of uranyl (uranium ions).
  • To evaluate the probe's efficacy in biological samples and living organisms for assessing internal uranium contamination.
  • To establish a tool for immediate medical care of uranium-toxic individuals.

Main Methods:

  • Design and synthesis of a near-infrared (NIR) fluorescent probe (NRhB-AO) incorporating an amidoxime group for uranyl selectivity.
  • Utilizing fluorescence spectroscopy to quantify uranyl-induced signal enhancement.
  • Employing Density Functional Theory (DFT) calculations to elucidate the uranyl binding mechanism.
  • Testing the probe's performance in cellular assays (NRK-52E cells) and in vivo imaging of uranyl-exposed mice.

Main Results:

  • The NRhB-AO probe exhibited a remarkable 71.5-fold fluorescence enhancement upon binding with uranyl.
  • DFT calculations confirmed uranyl chelation induces spirolactam ring-opening, leading to fluorescence increase via a 1:1 complex.
  • Rapid and quantitative intracellular uranyl detection was achieved in NRK-52E cells.
  • In vivo imaging revealed significant signal enhancement in the kidneys of uranyl-exposed mice, indicating rapid uptake.

Conclusions:

  • The NRhB-AO fluorescent probe is highly effective for sensitive uranyl detection in biological environments.
  • This probe facilitates noninvasive, rapid, and accurate evaluation of internal uranium contamination.
  • The developed method holds significant potential for improving the medical management of uranium toxicity.