Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)00:53

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
Step-Growth Polymerization: Overview01:03

Step-Growth Polymerization: Overview

Step-growth or condensation polymerization is a stepwise reaction of bi or multifunctional monomers to form long-chain polymers. As all the monomers are reactive, most of the monomers are consumed at the early stages of the reaction to form small chains of reactive oligomers, which then combine to form long polymer chains in the late stages. Hence, the reaction has to proceed for a long time to achieve high molecular weight polymers.
Many natural and synthetic polymers are produced by...
Ziegler–Natta Chain-Growth Polymerization: Overview01:17

Ziegler–Natta Chain-Growth Polymerization: Overview

Ziegler–Natta polymerization is another form of addition or chain‐growth polymerization used for synthesizing linear polymers over branched polymers. The catalyst used for polymerization is the Ziegler–Natta catalyst, named after Karl Ziegler and Giulio Natta, who developed it in 1953. This catalyst is an organometallic complex of titanium tetrachloride and triethyl aluminum, with the active form of the catalyst being an alkyl titanium compound. Using the Ziegler–Natta catalyst, high molecular...
Anionic Chain-Growth Polymerization: Overview01:20

Anionic Chain-Growth Polymerization: Overview

The polymerization process that involves carbanion as an intermediate is called anionic polymerization. It is also a type of addition or chain-growth polymerization. Anionic polymerization gets initiated by a strong nucleophile such as an organolithium or a Grignard reagent. The most commonly used initiator for anionic polymerization is butyl lithium. Monomers involved in anionic polymerization must possess a vinyl group bonded to one or two electron-withdrawing groups. For instance,...
Olefin Metathesis Polymerization: Overview01:13

Olefin Metathesis Polymerization: Overview

Recently, the development of olefin metathesis polymerization advanced the field of polymer synthesis. Simply put, the reorganization of substituents on their double bonds between two olefins in the presence of a catalyst is known as the olefin metathesis reaction. The use of metathesis reaction for polymer synthesis is called olefin metathesis polymerization.
Ruthenium-based Grubbs catalyst is the most commonly used catalyst for olefin metathesis polymerization. Grubbs catalyst consists of a...
ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genuine Directed Evolution In Test Tube (GENie).

bioRxiv : the preprint server for biology·2026
Same author

MagNanoTrap Enrichment Empowers Ultra-Sensitive Quantification of Mixed Nanoplastic Particles From Environmental Water Samples.

Angewandte Chemie (International ed. in English)·2026
Same author

Azidocoumarin Glycan Probes for Photoinduced Cross-Linking and In Situ Fluorescent Labeling.

Bioconjugate chemistry·2026
Same author

Conformation-Sensitive Active-Site Residue Tyrosine 260 in <i>Paenibacillus macerans</i> Cyclodextrin Glycosyltransferase Governs C13-Regioselective Glycosylation of Rebaudioside A.

Journal of agricultural and food chemistry·2026
Same author

<i>De Novo</i> Design of High-Performance Sec-type Signal Peptide via a Hybrid Deep Learning Architecture.

JACS Au·2025
Same author

Diacetylene-Functionalized Glycan Mimetics for Receptor-Mediated Cluster Imprinting in Model Membranes.

Macromolecular rapid communications·2025

Related Experiment Video

Updated: Jun 10, 2026

Depolymerizable Olefinic Polymers Based on Fused-Ring Cyclooctene Monomers
08:12

Depolymerizable Olefinic Polymers Based on Fused-Ring Cyclooctene Monomers

Published on: December 16, 2022

Defined Nylon Oligomers Enable Mechanistic Insight Into Enzymatic Polyamide Depolymerization.

Sukmanita Dewi1, Hendrik Puetz2, Ulrich Schwaneberg2

  • 1Institute of Macromolecular Chemistry, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.

Chemsuschem
|June 9, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to create nylon oligomers for studying enzymatic degradation. This breakthrough aids in understanding biocatalysis for sustainable nylon recycling.

Keywords:
enzymatic polymer degradationnylon oligomerspolyamide depolymerizationsequence‐defined oligomerssolid‐phase synthesis

More Related Videos

Controlled Photoredox Ring-Opening Polymerization of O-Carboxyanhydrides Mediated by Ni/Zn Complexes
05:48

Controlled Photoredox Ring-Opening Polymerization of O-Carboxyanhydrides Mediated by Ni/Zn Complexes

Published on: November 21, 2017

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Related Experiment Videos

Last Updated: Jun 10, 2026

Depolymerizable Olefinic Polymers Based on Fused-Ring Cyclooctene Monomers
08:12

Depolymerizable Olefinic Polymers Based on Fused-Ring Cyclooctene Monomers

Published on: December 16, 2022

Controlled Photoredox Ring-Opening Polymerization of O-Carboxyanhydrides Mediated by Ni/Zn Complexes
05:48

Controlled Photoredox Ring-Opening Polymerization of O-Carboxyanhydrides Mediated by Ni/Zn Complexes

Published on: November 21, 2017

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Area of Science:

  • Polymer Chemistry
  • Biocatalysis
  • Enzymology

Background:

  • Enzymatic polyester recycling shows promise for sustainability.
  • Enzymatic polyamide (PA) degradation is poorly understood due to a lack of suitable model substrates.
  • Developing defined substrates is crucial for advancing biocatalytic PA recycling.

Purpose of the Study:

  • To develop a robust synthesis strategy for defined, monodisperse nylon-6 and nylon-6,6 oligomers.
  • To create a standardized library of nylon oligomers as model substrates for amidase screening.
  • To enable mechanistic investigations into enzyme-nylon interactions for biocatalytic polyamide depolymerization.

Main Methods:

  • Solid-phase synthesis strategy for generating nylon-6 and nylon-6,6 oligomers with adjustable chain lengths.
  • Characterization of synthesized oligomers for solubility and analytical traceability.
  • Screening of a representative amidase using the synthesized nylon oligomer library.

Main Results:

  • Successfully synthesized defined, monodisperse nylon oligomers mimicking bulk polyamides.
  • Demonstrated the utility of the oligomer library for screening amidase activity.
  • Observed distinct chain length-dependent conversion profiles, matching bulk material degradation patterns.

Conclusions:

  • The synthetic strategy provides a critical bridge between polymer chemistry and enzymatic screening for polyamides.
  • The developed nylon oligomers serve as realistic surrogates for studying biocatalytic polyamide depolymerization.
  • This work lays the foundation for rational exploration of enzymatic nylon recycling.