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Updated: Jun 10, 2026
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Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
Published on: May 10, 2024
Enhancing Preclinical Rigor: Evaluating Robustness and Numerical Stability in a Chronic Pancreatitis Mouse Model
Annika Thämlitz1, Muhammad Imran Khan1, Benjamin Schulz1
1Institute for Experimental Surgery, University Medical Centre Rostock, Rostock, Germany.
Annals of the New York Academy of Sciences
|June 9, 2026
Summary
This study validates a simplified chronic pancreatitis (CP) mouse model, demonstrating its reliability in replicating key disease hallmarks. The RORγt inhibitor GSK805 showed limited therapeutic effects in this robust CP model.
Area of Science:
- Gastroenterology
- Immunology
- Toxicology
Background:
- Chronic pancreatitis (CP) involves progressive fibrosis, inflammation, and pancreatic atrophy.
- Developing reliable animal models is crucial for understanding CP pathogenesis and testing therapeutics.
Purpose of the Study:
- To evaluate a simplified cerulein-induced chronic pancreatitis (CP) mouse model.
- To assess the therapeutic potential of the RORγt inhibitor GSK805 in this CP model.
- To validate the model's reliability using statistical metrics like robustness of conclusion (RC).
Main Methods:
- Administration of 42 cerulein injections to male and female C57BL/6J (BL6) and BALB/c mice.
- Evaluation of CP hallmarks: pancreas-to-body weight ratio, collagen I deposition, and tubular complex formation.
- Assessment of inflammatory gene expression (Tnf, Ifng, Il10, Il23r, Il6, Il1b) and statistical robustness.
Main Results:
- The CP model reliably reproduced key pathological features with 100% robustness of conclusion (RC).
- Inflammatory gene induction varied by mouse strain and sex; Il23r and Il10 were induced in BL6 mice, while Il6 was induced in BALB/c mice.
- GSK805 treatment significantly reduced Il23r expression in BL6 mice, but did not improve other CP characteristics.
Conclusions:
- A simplified cerulein-induced CP model is robust and reliable for studying pancreatitis.
- Inflammatory responses in CP models can be strain- and sex-specific.
- The RORγt inhibitor GSK805 demonstrated limited efficacy in this CP model, highlighting the need for further therapeutic research.
