Diesel biodegradation by biosurfactant producing Paenibacillus strains

This study addresses the need for eco-friendly and cost-effective solutions to remediate petroleum-contaminated soils. It explores the untapped potential of biosurfactant producing Paenibacillus strains for bioremediation. Eight Paenibacillus strains procured from NCMR (NCCS), Pune, were evaluated for biosurfactant production as well as hydrocarbon degradation. Paenibacillus chitinolyticus, P. borealis and P. castaneae showed high emulsification activity (EA > 240 EU/ml) and emulsification index (EI > 30%), while P. castaneae, P. chitinolyticus, P. alvei and Paenibacillus sp. 2 (MCC 2683) reduced surface tension below 40 mN/m. To the best of our knowledge, this is the first report on biosurfactant production and hydrocarbon biodegradation by Paenibacillus chitinolyticus, Paenibacillus castaneae and their consortium. A bacterial consortium of P. chitinolyticus and P. castaneae was optimized for diesel degradation using response surface methodology, achieving 34.63% degradation in 10 days of treatment under optimal conditions (37 °C, 5% diesel, 5% inoculum, pH 6.5). Laboratory-scale soil bioremediation over three months yielded 44.78% diesel degradation, confirmed by gas chromatography. These findings highlight the consortium's potential for environmental bioremediation.