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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Jun 11, 2026

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
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Published on: August 2, 2011

Performance Comparison of Commercially Available Kits for Wild Birds RNA Extraction.

Ane López-Morales1, Vega Alvarez2, Xeider Gerrikagoitia3

  • 1Basque Research and Technology Alliance (BRTA), Animal Health Department. NEIKER -Basque Institute for Agricultural Research and Development, Derio, Spain; ane.lopez@neiker.eus.

Canadian Journal of Microbiology
|June 9, 2026
PubMed
Summary

Choosing the right RNA extraction kit is crucial for wildlife studies. The RNeasy PowerFecal Pro Kit generally performed best for wild bird samples, offering higher RNA quality and less inhibition.

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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Area of Science:

  • Wildlife biology
  • Molecular biology
  • Biotechnology

Background:

  • RNA stability is a challenge in environmental samples, especially from free-ranging animals.
  • Effective RNA extraction is vital for molecular analyses in wildlife research.

Purpose of the Study:

  • To assess the performance of three commercial RNA extraction kits.
  • To compare RNA yield, integrity, and purity across different wild bird species and sample types.

Main Methods:

  • Extraction of RNA from 24 wild bird samples (faeces, oropharyngeal, cloacal swabs) using three Qiagen kits.
  • Evaluation of RNA yield, integrity, and purity (260/280, 260/230 ratios).
  • Assessment of sample inhibition and statistical modeling using multiple linear regression.

Main Results:

  • RNA extraction efficiency varied significantly by species, sample type, and kit.
  • The RNeasy PowerFecal Pro Kit demonstrated superior RNA integrity and purity, particularly for faecal samples.
  • This kit also exhibited reduced reverse transcription quantitative polymerase chain reaction (RT-qPCR) inhibition.

Conclusions:

  • Kit selection is critical and depends on the specific wildlife species and sample matrix.
  • The RNeasy PowerFecal Pro Kit is recommended for challenging wildlife samples, especially faeces.
  • Optimized RNA extraction protocols enhance the reliability of molecular data from wild populations.