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Updated: Jun 11, 2026
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Engineering an amino-modified MOF@COF interface to construct a light-responsive oxidase-like nanozyme for rapid total
Yanhui Feng1, An Xu1, Huan Li1
1College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, PR China.
Abstract:
A rapid and accurate assay for total antioxidant capacity (TAC) is essential in food quality control and nutritional evaluation. In this study, a light-responsive oxidase-like nanozyme, UiO-66-NH2@TpPa-COF, was constructed by in-situ growth of TpPa-COF on amino-functionalized UiO-66-NH2. Comprehensive characterization confirmed a tightly coupled, crystalline, and hierarchically porous heterostructure with a narrowed band gap and enhanced charge transfer. Under 365 nm light, the composite exhibited efficient photoelectrochemical performance and oxidase-like activity via a light-driven, oxygen-dependent reactive oxygen species pathway. As a colorimetric TAC (μM AA Eq.) sensor, it achieved a wide linear range (0.02-60 μM for AA), a low detection limit (5.988 nM), and reliable performance in real samples (fruits, vegetables, beverages) with recoveries of 96-107%. This work provides both a sensitive nanozyme-based sensing platform and insights into the rational design of MOF/COF hybrids for advanced food analysis.
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