Development of a quantum dot-based immunochromatographic test strip for rapid detection of infectious bursal disease...

Infectious bursal disease (IBD) is a highly contagious avian disease caused by infectious bursal disease virus (IBDV), mainly affecting the bursa of Fabricius immune organ in young chickens. The virus has heat and acid resistance and can be spread through contaminated environments, feed and insects, leading to immunosuppression and increasing the risk of secondary infections. Affected chickens often show symptoms such as diarrhea, tremors and dehydration. Chickens aged 3-6 weeks are the most susceptible, with an incidence rate of up to 100% and a mortality rate of 5-30%. Therefore, from the perspective of infection control, effective monitoring and prevention of IBDV are of vital importance. The quantum dot fluorescence immunochromatographic strip established in this study can complete the specific detection of infectious bursal disease in chickens within 15 min, with a detection limit of 10^2.5 TCID₅₀/mL for infectious bursal disease virus liquid. It does not cross-react with avian influenza viruses (H9N2, H10N8) and infectious bronchitis virus. This test strip can rapidly and accurately detect infectious bursal disease virus, providing a valuable tool for on-site detection.